Stably-expressing Cell Line Production in 28 Days

Altogen Biosystems offers contract research services including: generation of stable cell lines, vector construction, and RNAi cell transfection services. Generation of cells permanently expressing gene of interest or siRNA-expressing construct can be very complex, expensive and time-consuming. Altogen Custom Services utilize their unique experience with over hundred cancer cell lines and expertise in polymer and nanoparticle-based gene delivery technologies to produce stably-expressing clonal cells in just 28 days. Other custom services include assay development, cloning, transient transfection, shRNA-encoding vector construction, siRNA design and validation in vitro.

In Vivo Transfection Reagents

New Generation In Vivo Transfection Reagents

Altogen Biosystems offers various types of in vivo transfection reagents used for testing pharmaceuticals in animals. The major challenge in performing RNAi studies in vivo is the efficient, directed delivery of functional siRNA, shRNA, and microRNA molecules into specific tissues. Lipid-, polymer-, and nanoparticle-based in vivo transfection reagents provides differential transfection efficiency to particular organs and tumor tissues. All in vivo transfection reagents are stable in serum, enhance delivery efficiency, and provide prolonged siRNA-induced gene silencing effect. Reagents applicable for systemic administration, as well as direct intratumoral and intraperitoneal injections.

Pre-optimized Transfection Reagents

Cell Line Specific Transfection Reagents

Altogen Biosystems is a leading manufacturer and developer of cell type specific, pre-optimized transfection kits and products. Reagents are designed for individual cancer cell lines and many primary cell types, and are capable of delivering functional plasmid DNA, mRNA, siRNA, and microRNA molecules into cells. Most DNA transfection reagents are incompatible with siRNA, sensitive to serum, or are capable of inducing cytotoxicity, which negatively affects gene expression studies. ALTOGEN® Transfection Reagents have been developed and optimized specifically for use with siRNA transfection and allow transfection in the presence of serum without lowering transfection efficiency or reducing cell viability. Ready-to-run transfection protocols eliminate the need for extensive transfection optimization experiments. Transfection kits include cell type specific transfection protocols and transfection enhancer reagent. Transfection kits are highly efficient, cost-effective, cell type specific, reverse transfection compatible, and provide high reproducibility.

Transfection Resource

siRNA Transfection || Transfection Methods || Stable Transfection || RNAi || siRNA Library Screening || RNAi Therapeutics || Cell Transfection || In Vivo Transfection Reagents || Cell Line Specific Transfection Reagents

Featured Products

In Vivo Transfection Reagents || Fibroblast Cells Transfection Reagent || Astrocyte Transfection Reagent || HEK-293 Transfection Reagent || MEF Transfection Reagent || HepG2 Transfection Reagent || CHO Reagent || MDA-MB Transfection Reagent || Cos7 Transfection Reagent || A549 Transfection Reagent || NIH3T3 Reagent || HUVEC Transfection Reagent || LNCaP Transfection Reagent || MCF-7 Reagent || PC-12 Reagent

Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.