VERO Transfection Reagent

Catalog # 3281 - 0.5 mL        $ 149.00 Catalog # 3282 - 1.5 mL        $ 231.00 Transfection reagent for VERO cells Kit includes Transfection Enhancer reagent and recommended transfection protocols Protocols provided for transfection of siRNA, microRNA and mRNA High transfection efficiency Work for reverse transfection and high throughput protocols Compatible with transfection in any plate format Reproducible transfection results Minimal off-target effects Developed and manufactured by Altogen Biosystems Transfection Efficiency: Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR. Transfection Protocol: Transfection protocols are provided with the purchase of the reagent.

DATA

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

VERO cell line

The Vero lineage was isolated from kidney epithelial cells extracted from Cercopithecus aethiops (African green monkey). The lineage was developed by Yasumura and Kawakita in 1962. The original cell line was named "Vero" from "Verda Reno" (green kidney in esperanto). Vero cells are used for multiple purposes, including screening for the toxins, as a host cell line for growing virus, as a host cell line for eukaryotic parasites (Trypanosomatids.) The Vero cell lineage is aneuploid and continuous. It can be replicated through numerous cycles of division without becoming senescent. Aneuploidy means having an abnormal number of chromosomes. Unlike normal mammalian cells Vero cells are interferon-deficient. They don’t produce type 1 interferons when infected. For more information on Fibroblast cells visit Wikipedia.

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Altogen Research Services

Altogen Custom Services provide specialized biotechnology and pharmaceutical services, including - generation of stable cell lines, RNA Interference (RNAi) services, assay development, screening and transfection services. Generation of stably-expressing cancer cell lines and primary cells can be very expensive and time-consuming. Altogen Services offers generation of stable cell lines by transforming the cell line of choice to stably express gene of interest or shRNA-encoding construct. Cloning and shRNA expression services are available.