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Transfection Reagent for Detroit-551 Cells (Rhabdomyosarcoma Cells)
A biodegradable polymer based transfection agent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
Achieve robust siRNA uptake for dependable gene silencing
Effective transfection under conditions of up to 40% serum
Gentle enough to be used for single cell analysis
- Download PowerPoint presentation for Detroit-551 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 78% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems Detroit-551 Transfection Protocol: [PDF]
Download MSDS: [PDF]
Detroit-551 Cell Line:
The Detroit 551 cell line was originally established in 1965 using skin tissue taken from the relatively healthy fetus of a Caucasian female. These cells exhibit a fibroblast morphology and cell type. Detroit 551 cells are also useful for research purposes because of their ability to have their growth enhanced by the presence of TNF (tumor necrosis factor) alpha. This cell line can be transformed by SV40, and have a lifespan of ~25 passages.
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into Detroit-551 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in Detroit-551 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into Detroit-551 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems provides pre-optimized transfection kits and electroporation products for life science research. Transfection products are developed for individual cancer cell line and transfection protocols are optimized to enable high transfection efficiency of biomolecules. Altogen Biosystems developed in vivo delivery products for small animal research, mouse and rat targeted tissue delivery: liver targeted, pancreas targeted, kidney targeted, PEG-Liposome-, Nanoparticle-, Lipid-, and Polymer-based in vivo transfection kits. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of biomolecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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