HCS-2 Transfection Kit (Chondrosarcoma Cells)

$236.00$1,354.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #1761)$236.00
1.5 ml (Catalog #1762)$518.00
1.5 ml CRISPR (Catalog #2147)$718.00
8.0 ml (Catalog #7044)$1,354.00
SKU: HCS2 Category:

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

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Transfection Reagent for HCS-2 Cells (Chondrosarcoma Cells)

  • High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
  • Kit includes Transfection Enhancer reagent
  • Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
  • Work in the presence of serum
  • Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
  • Download in vitro HCS2 transfection protocol: [PDF]
  • Download HCS2 CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for HCS2 cells transfection kit: [PPT]
  • Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems HCS-2 Transfection Protocol: [PDF]

Download MSDS: [PDF]

HCS-2 Cell Line:

Chondrosarcoma is a form of bone cancer that affects cartilage-producing cells of the human body, representing roughly 30 percent of all skeletal system tumors. It mostly affects the axial skeleton of adults ages 20 to 60, with approximately 600 new cases diagnosed annually in the United States, according to the Atlas of Genetics and Cytogenetics in Oncology and Haematology. The HCS-2 cell line was established from human chondrosarcoma cells and is widely used in cartilage research. Medical studies for patients with certain types of sarcoma (cancer) often employ this cell line as a biological model. Altogen Biosystems provides an advanced formulation of a lipid-based HCS-2 reagent kit for chondrosarcoma cells that yields high transfection efficiency with the HCS-2 cell line, a sufficient host for biomedical research related to chondrosarcoma and cartilage. Also, the HCS-2 transfection kit is commonly employed in gene expression studies and drug discovery.

Mutations:

PIK3CA 5290 37 3 178952085 178952085 Missense_Mutation SNP A G
MB21D2 151963 37 3 192516720 192516720 Missense_Mutation SNP G C
TP53 7157 37 17 7578176 7578176 Splice_Site SNP C T
ADAMTSL3 57188 37 15 84651117 84651117 Missense_Mutation SNP C T
VAV1 7409 37 19 6825377 6825377 Missense_Mutation SNP G A
DLGAP3 58512 37 1 35370062 35370062 Missense_Mutation SNP G A
UNC13C 440279 37 15 54307601 54307601 Missense_Mutation SNP G A
CD200 4345 37 3 112066436 112066436 Missense_Mutation SNP C A
CDH12 1010 37 5 21752319 21752319 Nonsense_Mutation SNP G A
GPR98 84059 37 5 90087051 90087051 Missense_Mutation SNP G A
HOXA6 3203 37 7 27185363 27185363 Missense_Mutation SNP G A

Data:

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the HCS-2 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).

HCS2-cells-transfection-protocol

Figure 2. Protein expression of Cyclophilin B in HCS-2 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into HCS-2 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits and reagents. Advanced formulation of reagents and optimized transfection protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #1761)
  • 1.5 ml (Catalog #1762)
  • 1.5 ml CRISPR (Catalog #2147)
  • 8.0 ml (Catalog #7044)

Additional information

Weight 1 lbs
Kit Size (Volume)

0.5 ml (Catalog #1761), 1.5 ml (Catalog #1762), 1.5 ml CRISPR (Catalog #2147), 8.0 ml (Catalog #7044)