Description
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Product Availability: In Stock.
Protein Electroporation Buffer (30 ml)
- Certified RNAse-, DNAse-free electroporation buffer
- Optimized for efficient protein transfer into any cell line or primary cell types
- Buffer formulation mimics cellular cytoplasm composition enhancing electroporation pore resealing
- Validated for use with human, rodent, and insect cell lines
- High viability and cell recovery rates
- Developed and manufactured by Altogen Biosystems
Protein Electroporation Buffer
The protein electroporation buffer is a specialized buffer used to prepare cells for electroporation with proteins. The buffer is designed to maintain the integrity of the cell membrane during the electroporation process, while also facilitating the uptake of the protein of interest.
The composition of the protein electroporation buffer may vary depending on the cell type and the protein being delivered, but typically includes the following components:
- Low salt concentration: A low salt concentration is used to minimize the osmotic shock to the cells during electroporation.
- Neutral pH: The buffer is adjusted to a neutral pH to maintain the stability of the protein of interest and prevent damage to the cells.
- Supplemental agents: Additional agents, such as sucrose or glycerol, may be added to the buffer to protect the cells and the protein from the effects of electroporation.
- Antibiotics: Antibiotics may be added to the buffer to prevent contamination of the cells with bacteria or fungi.
It is important to optimize the composition and concentration of the protein electroporation buffer to ensure efficient delivery of the protein into the cells while minimizing toxicity or other adverse effects. Careful attention should be given to the preparation of the buffer and the handling of the cells to ensure the success and reproducibility of the experiment.
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 30 ml (Catalog #4068)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.