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Transfection Reagent for Chromaffin Cells
- A biodegradable polymer based transfection reagent
- Once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
- High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
- Achieve robust siRNA uptake for dependable gene silencing
- Effective transfection under conditions of up to 20% serum
- Gentle enough to be used for single cell analysis
- Download Chromaffin CRISPR/Cas9 transfection protocol: [PDF]
- Download PowerPoint presentation for Chromaffin cells transfection kit: [PPT]
- Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 75% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems Chromaffin Transfection Protocol: [PDF]
Download MSDS: [PDF]
Chromaffin Cell Line:
Chromaffin cells are located in the medulla of the adrenal glands in mammals and are responsible for several functions, such as monitoring carbon dioxide and oxygen levels in the body, responding to stress or regulating the blood pressure. Closely related to the nervous system, chromaffin cells are one of the most commonly studied neural crest derivatives due to the variety of bioactive substances they secrete into the bloodstream, including adrenaline, noradrenaline, and other hormones. Adrenal glands are endocrine glands that secrete hormones into the bloodstream, to regulate the body’s response to stressful stimuli. Adrenal medulla, the central region of these glands, contains the cells that synthesize and secrete these hormones. Neuroendocrine cells release different hormones into the bloodstream in response to nerve signals. Chromaffin cells are mainly the activation site of the body’s flight or fight response. The chromaffin cell line was derived from human chromaffin cell cultures and is also known as medullary chromaffin cells situated in the sympathetic nervous system. “Chromaffin” is a combination of the words “chromium” and “affinity”; they were given this name because they easily pick up chromatin stain. In addition to being useful in the fields of cellular and molecular biology, chromaffin cells are valuable for stem cell research and clinical transplantation. Altogen Biosystems provides advanced nanoparticle-based liposome formulated chromaffin transfection reagent kits for human chromaffin cell lines that play an essential role in biomedical research. The reagent kits are highly sensitive and have exhibited low cytotoxicity.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in Chromaffin cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).
Figure 2. Protein expression of Cyclophilin B in Chromaffin cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into Chromaffin cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems provides optimized transfection kits and electroporation products for life sciences and cancer research. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits (animal transfection): 1) Tissue-targeted reagents (delivery of proteins, DNA, and RNA into liver, pancreas, or kidney tissues), and 2) Broad range in vivo biodistribution reagents (PEG-Liposome based reagent, Nanoparticle-based in vivo reagent, Lipid-based transfection kit, and Polymer-based kit). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged biomolecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP compliant contract research studies for preclinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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