Raji Electroporation Kit (Burkitts Lymphoma, CCL-86)

$394.00$796.00

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SKU: RAJI Category:

Description

Product Availability: In Stock (FedEx delivery within 2 business days).

Ordering: To place an order please use Add to Cart button (account not required).

Electroporation Kit for Raji Cells (Burkitt’s Lymphoma Cells, CCL-86)

• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems

Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm electroporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm electroporation cuvette

Electroporation Efficiency:
Electroporation kit provides 75-90% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.

Electroporation Protocol and MSDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download MSDS: [PDF]

Raji Cell Line:
The Raji cell line was established in 1963 from an 11-year-old black male patient who suffered from Burkitt’s lymphoma. It was isolated by R.J.V. Pulvertaft from B-lymphocytes, which were surgically removed from the patient’s body. This cell line is EBNA positive, and is somewhat resistant to the viruses such as polio and vesicular stomatitis, making it uniquely useful as a transfection host in molecular and cell biology research.

Raji cell line mutations:

TP53 7157 37 17 7577581 7577581 Missense_Mutation SNP A G
HRG 3273 37 3 186394974 186394974 Nonsense_Mutation SNP G T
FAM49A 81553 37 2 16743285 16743285 Missense_Mutation SNP G C
MYC 4609 37 8 128750639 128750639 Missense_Mutation SNP C T
PIK3C2G 5288 37 12 18716344 18716344 Missense_Mutation SNP G A
HERC2 8924 37 15 28386775 28386775 Splice_Site SNP G T
KIF27 55582 37 9 86504110 86504110 Missense_Mutation SNP C T
DOCK9 23348 37 13 99520515 99520515 Silent SNP G A
GLI2 2736 37 2 121740422 121740422 Missense_Mutation SNP G A
TTN 7273 37 2 179576811 179576811 Missense_Mutation SNP G A
SLC12A5 57468 37 20 44675056 44675056 Missense_Mutation SNP C T
RP1 6101 37 8 55534024 55534024 Silent SNP G T
SULT1A2 6799 37 16 28606983 28606983 Silent SNP C T
NAPSA 9476 37 19 50864331 50864331 Missense_Mutation SNP C T

 

Data:
Raji Electroporation Kit

Altogen Biosystems:

Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits. Advanced formulation of reagents and optimized electroporation protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about electroporation technology at Altogen’s Transfection Resource.

Altogen Research Services:

Altogen Labs provides good laboratory practice (GLP) compliant preclinical research services for IND applications and drug development. Our biology CRO services includes both efficacy studies (over 90 in-house validated xenograft models) and safety pharmacology/toxicology studies (for more details please visit AltogenLabs.com).

Volume Options:

  • 4 ml (Catalog #6581)
  • 30 ml (Catalog #6582)

Additional information

Weight 1 lbs
Kit Size (Volume)

4 ml (Catalog #6581), 30 ml (Catalog #6582)