Raji Electroporation Kit (Burkitts Lymphoma, CCL-86)

Description

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Electroporation Kit for Raji Cells (Burkitt’s Lymphoma Cells, CCL-86)

• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems

Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm electroporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm electroporation cuvette

Electroporation Efficiency:
Electroporation kit provides 75-90% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.

Electroporation Protocol and MSDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download MSDS: [PDF]

Raji Cell Line:
The Raji cell line was established in 1963 from an 11-year-old black male patient who suffered from Burkitt’s lymphoma. It was isolated by R.J.V. Pulvertaft from B-lymphocytes, which were surgically removed from the patient’s body. This cell line is EBNA positive, and is somewhat resistant to the viruses such as polio and vesicular stomatitis, making it uniquely useful as a transfection host in molecular and cell biology research.

Raji cell line mutations:

TP53	7157	37	17	7577581	7577581	Missense_Mutation	SNP	A	G
HRG	3273	37	3	186394974	186394974	Nonsense_Mutation	SNP	G	T
FAM49A	81553	37	2	16743285	16743285	Missense_Mutation	SNP	G	C
MYC	4609	37	8	128750639	128750639	Missense_Mutation	SNP	C	T
PIK3C2G	5288	37	12	18716344	18716344	Missense_Mutation	SNP	G	A
HERC2	8924	37	15	28386775	28386775	Splice_Site	SNP	G	T
KIF27	55582	37	9	86504110	86504110	Missense_Mutation	SNP	C	T
DOCK9	23348	37	13	99520515	99520515	Silent	SNP	G	A
GLI2	2736	37	2	121740422	121740422	Missense_Mutation	SNP	G	A
TTN	7273	37	2	179576811	179576811	Missense_Mutation	SNP	G	A
SLC12A5	57468	37	20	44675056	44675056	Missense_Mutation	SNP	C	T
RP1	6101	37	8	55534024	55534024	Silent	SNP	G	T
SULT1A2	6799	37	16	28606983	28606983	Silent	SNP	C	T
NAPSA	9476	37	19	50864331	50864331	Missense_Mutation	SNP	C	T

 

Data:

Altogen Biosystems:

Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits. Advanced formulation of reagents and optimized electroporation protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about electroporation technology at Altogen’s Transfection Resource.

Altogen Research Services:

Altogen Labs provides good laboratory practice (GLP) compliant preclinical research services for IND applications and drug development. Our biology CRO services includes both efficacy studies (over 90 in-house validated xenograft models) and safety pharmacology/toxicology studies (for more details please visit AltogenLabs.com).

Volume Options:

• 4 ml (Catalog #6581)
• 30 ml (Catalog #6582)