Caco-2 Transfection Reagent (Colon Carcinoma Cells)

$245.00$1,336.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #6347)$245.00
1.5 ml (Catalog #6348)$512.00
1.5 ml CRISPR (Catalog #2122)$712.00
8.0 ml (Catalog #6349)$1,336.00
SKU: CACO2 Category:

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required).


Transfection Reagent for Caco-2 Cells (Colon Adenocarcinoma Cell, HTB-37)

  • A biodegradable polymer based trasfection agent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
  • Optimized for intracellular delivery of plasmid DNA, si/shRNA, microRNA, and mRNA
  • High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
  • Achieve robust siRNA uptake for dependable gene silencing
  • Effective transfection under conditions of up to 40% serum
  • Gentle enough to be used for single cell analysis
  • Download in vitro Caco-2 transfection protocol: [PDF]
  • Download Caco-2 CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for Caco-2 cells transfection kit: [PPT]
  • UPC/GTIN/EAN: 860002089738
  • Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems
Transfection Efficiency:

Reagent exhibits at least 94% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems Caco2 Transfection Protocol: [PDF]

Download MSDS: [PDF]

Caco-2 Cell Line:

The earlier colon cancer is diagnosed, the better the outlook for complete recovery and survival. However, despite the recent cancer breakthroughs, it can be extremely challenging to increase life expectancy in patients with late-stage colon cancer. The Caco-2 cell line is established from a patient with colorectal carcinoma and is widely known for its rapid differentiating ability. With a modal chromosome number of 96, many of these cells are polyploidy. The Caco-2 cell line is susceptible to human immunodeficiency virus 1 and could be potentially useful for the study of certain types of colon cancer in humans. This cell line has been known to double every ~62 hours. Transfection is a powerful investigative research tool that can be used to study molecular and biochemical pathways in cancer cells. Altogen Biosystems provides efficient biodegradable transfection reagent kits for the Caco-2 colon cancer cell line. A polymer-based transfection Caco-2 kit for colon adenocarcinoma cells yields at least 94% transfection efficiency and is a helpful tool for gene expression studies as well as for assessing cellular functionality.

Mutations:

CCNL2 81669 37 1 1326204 1326204 Missense_Mutation SNP C T
RERE 473 37 1 8421855 8421855 Missense_Mutation SNP C A
RP11-268J15.5 0 37 1 36793521 36793521 Silent SNP C T
RP11-268J15.5 0 37 1 36793522 36793522 Missense_Mutation SNP C T
ZYG11A 440590 37 1 53351510 53351510 Silent SNP A G
JAK1 3716 37 1 65330523 65330523 Missense_Mutation SNP T C
SLC44A5 204962 37 1 75708571 75708571 Splice_Site SNP C T
NBPF6 653149 37 1 109010281 109010281 Silent SNP T C
TSPAN2 10100 37 1 115593140 115593140 Missense_Mutation SNP C G
WDR3 10885 37 1 118502028 118502028 Silent SNP G A
GPR89B 51463 37 1 147416180 147416180 Missense_Mutation SNP G A
NBPF14 25832 37 1 148004663 148004663 Missense_Mutation SNP T C
GON4L 54856 37 1 155735986 155735986 Missense_Mutation SNP G C

Data:

Caco2 Transfection Reagent

Figure 1. GAPD mRNA levels were quantified using real-time qPCR in the Caco-2 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time qPCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).

Caco2-cells-transfection-protocol

Figure 2. Protein expression of GAPDH in Caco-2 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into Caco-2 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Selected Caco-2 transfection reagent citation references:

  • PLoS One. 2014 10;9(7). SOX2 expression is regulated by BRAF and contributes to poor patient prognosis in colorectal cancer. Lundberg et al [PDF]
  • Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
  • Gastroenterology. 2011 141(2) Differential type I interferon-mediated autophagic trafficking … Desai et al [PDF]
  • PLOS ONE. 2014. SOX2 Expression Is Regulated by BRAF and … Lundberg et al [PDF]

Altogen Biosystems:
Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and preoptimized transfection kits, electroporation buffers, and targeted in vivo delivery kits and reagents. Advanced formulation of reagents and optimized transfection protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP compliant contract research studies for preclinical research, IND applications, and drug development. Our biology CRO services include: Efficacy xenograft services (over 90 xenograft models), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (please visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6347)
  • 1.5 ml (Catalog #6348)
  • 1.5 ml CRISPR (Catalog #2122)
  • 8.0 ml (Catalog #6349)

Additional information

Weight 1 lbs
Kit Size (Volume)

0.5 ml (Catalog #6347), 1.5 ml (Catalog #6348), 1.5 ml CRISPR (Catalog #2122), 8.0 ml (Catalog #6349)