ZR-75-1 Transfection Kit (Breast Carcinoma, CRL1500)


Kit Size VolumePricePay with CC, PO, or PaypalAdd to Cart
0.5 ml (Catalog #6930)$392.00
1.5 ml (Catalog #6931)$786.00
1.5 ml CRISPR (Catalog #2203)$786.00
8.0 ml (Catalog #7094)$2,058.00
SKU: ZR751 Category:


Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.

Transfection Reagent for ZR-75 Cells (Breast Carcinoma Cells, CRL-1500)

  • Proprietary cationic lipids formulation
  • High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, and pDNA
  • A proven reagent for establishing stable cell lines
  • Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
  • Download in vitro ZR 751 transfection protocol: [PDF]
  • Download ZR751 CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for ZR-75-1 cells transfection kit: [PPT]
  • Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 93% transfection efficiency of siRNA delivery. Transfection efficiency was determined by RT-PCR

Transfection Protocol and MSDS:

Download Altogen Biosystems ZR 751 Transfection Protocol: [PDF]

Download MSDS: [PDF]

ZR-75-1 Cell Line:

The ZR-75-1 cell line is a human breast cancer cell line that was derived from a metastatic site of a patient with infiltrating ductal carcinoma in 1975. ZR-75-1 cells are commonly used in cancer research to study the biology of breast cancer and test novel therapies. Breast cancer is considered the most prevalent malignancy in females and the fifth most common cause of cancer-related fatalities among women worldwide, representing a significant public health challenge. Metastatic breast cancer is the primary cause of death in patients diagnosed with breast cancer. Preclinical cell line models could help in discovering innovative treatment options for those affected. The ZR-75 cell line is established from a 63-year-old Caucasian female with ductal carcinoma of the breast.  This cell line expresses an estrogen receptor and possesses a hypertriploid chromosome number (polyploidy). In addition to producing high levels of MUC-1 mucin mRNA, the ZR-75-1 cell line has a population doubling time of nearly 80 hours. ZR-75 is an epithelial cell line and is useful for studying the properties of tumors in breast cancer patients. Altogen Biosystems offers highly efficient pre-optimized transfection kits for the ZR-75-1 cell line.

Cell line mutations:

PTEN 5728 37 10 89692839 89692839 Missense_Mutation SNP T G
ARL2BP 23568 37 16 57282555 57282555 Splice_Site SNP C T
SIRPB1 10326 37 20 1552399 1552399 Missense_Mutation SNP G A
NAGA 4668 37 22 42463259 42463259 Silent SNP C T
CA6 765 37 1 9018981 9018981 Missense_Mutation SNP C T
CLCNKB 1188 37 1 16374426 16374426 Missense_Mutation SNP A G
EPHB2 2048 37 1 23235571 23235571 Silent SNP C T
CDCA8 55143 37 1 38158657 38158657 Silent SNP C T
GBP1 2633 37 1 89524716 89524716 Nonsense_Mutation SNP C A
RPAP2 79871 37 1 92789263 92789263 Missense_Mutation SNP C A


ZR75 Transfection Reagent

Figure 1. Cyclophilin B silencing efficiency was determined by RT-PCR in the ZR75 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).


Figure 2. Protein expression of Cyclophilin B in ZR-75-1 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into ZR-75-1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems provides preoptimized transfection kits and electroporation products for life science research. Transfection products are developed for individual cancer cell line and transfection protocols are optimized to enable high transfection efficiency of biomolecules. Altogen Biosystems developed in vivo delivery products for small animal research, mouse and rat targeted tissue delivery: liver targeted, pancreas targeted, kidney targeted, PEG-Liposome-, Nanoparticle-, Lipid-, and Polymer-based in vivo transfection kits. Advanced formulation of reagents and optimized transfection protocols provide efficient cellular delivery of charged molecules. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs CRO Services:

Altogen Labs provides good laboratory practice (GLP) compliant preclinical research services for IND applications and drug development. Our biology CRO services includes both efficacy studies (over 90 in-house validated xenograft models) and safety pharmacology/toxicology studies (for more details please visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6930)
  • 1.5 ml (Catalog #6931)
  • 1.5 ml CRISPR (Catalog #2203)
  • 8.0 ml (Catalog #7094)

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.

Additional information

Weight 1 lbs
Dimensions 4 × 3 × 0.2 in
Kit Size (Volume)

0.5 ml (Catalog #6930), 1.5 ml (Catalog #6931), 1.5 ml CRISPR (Catalog #2203), 8.0 ml (Catalog #7094)