NOTE: To place order using Credit Card, P-Card, or Purchase Order – please choose kit size above and click “Add to cart” button. You can place order online without creating an account.
Transfection Reagent for HCT-116 Cells (Colon Carcinoma Cells, CCL-247)
Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Kit includes Transfection Enhancer reagent
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
A proven reagent for establishing HCT-116 stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
Download PowerPoint presentation for HCT-116 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems HCT116 Transfection Protocol: [PDF]
Download MSDS: [PDF]
HCT-116 Cell Line:
The HCT-116 cell line was derived from epithelial tumorigenic colon tissue from an adult male who suffered from colorectal carcinoma. These cells have a mutation in the 13 codon of the ras protooncogene, and therefore they are useful for PCR assays studying this codon. They are also positive for transforming growth factor beta 1 and beta 2 expression. This cell line is useful for biomedical research related to colon cancer.
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into HCT-116 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in HCT-116 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into HCT-116 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, electroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
- 0.5 ml (Catalog #6811)
- 1.5 ml (Catalog #6812)
- 8.0 ml (Catalog #7045)