product_225

Calu-3 Transfection Kit (Lung Carcinoma, HTB-55)

$190.00$1,200.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #6401)$190.00
1.5 ml (Catalog #6402)$400.00
8.0 ml (Catalog #6403)$1,200.00
SKU: CALU3

Product Description

NOTE: To place order using Credit Card, P-Card, or Purchase Order – please choose kit size above and click “Add to cart” button. You can place order online without creating an account.

Transfection Reagent for Calu-3 Cells (Lung Carcinoma Cells, HTB-55)

  • Proprietary cationic lipids formulation
  • High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
  • Effective and robust intracellular delivery
  • Kit includes Transfection Enhancer reagent
  • Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
  • Work in the presence of serum
  • A proven reagent for establishing stable cell lines
  • Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
  • Download in vitro Calu-3 transfection protocol: [PDF]
  • Download PowerPoint presentation for Calu-3 cells transfection kit: [PPT]
  • Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems Transfection Protocol: [PDF]

Download MSDS: [PDF]

Calu-3 Cell Line:

The Calu-3 cell line was originally established from the lung tissue from a 25-year-old Caucasian male who suffered from adenocarcinoma. This patient was treated with Cytoxan, bleomycin, and Adriamycin prior to removal of cells. These tumorigenic cells exhibit an epithelial cellular morphology. Calu-3 could be useful for applications in transfection, or biomedical research on types of lung cancer. Calu-3 cells also useful as a transfection host cell line model of lung cancer.

Data:

Calu3 Cell Transfection Kit

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into Calu-3 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

calu3-cells-transfection-protocol

Figure 2. Protein expression of Lamin A/C in Calu-3 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into Calu-3 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:

Altogen Biosystems provides pre-optimized transfection kits and electroporation products for life science research. Transfection products are developed for individual cancer cell line and transfection protocols are optimized to enable high transfection  efficiency of biomolecules. Altogen Biosystems developed in vivo delivery products for small animal research, mouse and rat targeted tissue delivery: liver targeted, pancreas targeted, kidney targeted, PEG-Liposome-, Nanoparticle-, Lipid-, and Polymer-based in vivo transfection kits. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of biomolecules. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6401)
  • 1.5 ml (Catalog #6402)
  • 8.0 ml (Catalog #6403)

Additional Information

Kit Size (Volume)

0.5 ml (Catalog #6401), 1.5 ml (Catalog #6402), 8.0 ml (Catalog #6403)