Liver In Vivo Transfection Kit

$695.00$9,450.00

Kit Size VolumePricePay with CC, PO, or PaypalAdd to Cart
0.5 ml - 10 injections (Catalog #5060)$695.00
1.5 ml - 30 injections (Catalog #5061)$1,395.00
8.0 ml - 160 injections (Catalog #5062)$3,885.00
25 ml - 50 rat injections or 500 mouse injections (Catalog #5063)$9,450.00
SKU: LIVIV Category:

Description

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Liver In Vivo Transfection Reagent
siRNA and DNA liver in vivo delivery reagent for animal research (mouse, rat)

Modes of administration:

  • Systemic intravenous (i.v.) injection
  • Direct intratumoral (i.t.) injection
  • Intraperitoneal (i.p.) injection

Liver-targeted In Vivo Transfection Reagent

  • Biodegradable lipid liposome-based reagent
  • Liposome conjugated complexes are stable in serum for 16 hours
  • Efficient delivery to the liver tissue (via systemic administration) and liver tumors (via intratumoral administration)
  • Efficient siRNA, shRNA, miRNA, and plasmid DNA delivery
  • Minimal toxicity
  • Functionally validated in mice
  • Applicable for plasmid DNA/siRNA co-injection
  • Download Liver-targeted in vivo transfection protocol: [PDF] [Word]
  • Download PowerPoint presentation for Liver-targeted in vivo transfection kit: [PPT]
  • Download safety data sheet: [PDF]
  • UPC/GTIN/EAN: 860002089711
  • Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

DATA

In Vivo Liver Transfection Reagent Liver Hepatocarcinoma Tumors

Figure 1. Systemic administration (i.v.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Figure 2. Intratumoral administration (i.t.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Animal model is NOD/SCID xenograft mouse bearing flank tumor of human origin (liver cancer cell line). Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Liver-Targeted-Transfection-Altogen-Catalog-5062-4 Liver-Targeted-Transfection-Altogen-Catalog-5062-1 Liver-Targeted-Transfection-Altogen-Catalog-5062-2 Liver-Targeted-Transfection-Altogen-Catalog-5062-3

Figure 3. Systemic administration (i.v.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

Selected Liver InVivo Transfection Reagent citation references:

  • Molecular Therapy Nucleic Acids. 2017. Reverse Expression of Aging-Associated Molecules through Transfection … Kim H. et al [PDF]
  • PLoS Pathog. 2014 10(10) Exosomes from hepatitis C infected patients transmit HCV … Bukong et al [PDF]
  • Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
  • Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
  • J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]
  • J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]

Altogen Research Services:

Altogen Labs provides GLP compliant contract research services for IND applications and drug development. Our laboratory research services includes: Xenograft services (over 90 in-house validated xenograft models), development of stable cell lines (RNAi and protein production), ELISA assay development, cell-based and tissue targeted expression studies, safety pharmacology and toxicology assays, and many other preclinical research studies (visit AltogenLabs.com).

Liver-targeted delivery

There are several strategies for achieving liver-targeted delivery. One approach involves using a liver-specific promoter to drive expression of the gene of interest in liver cells. This can be achieved by incorporating the liver-specific promoter into the plasmid DNA or RNA construct that is being delivered. Another approach involves using a delivery vehicle that is specifically designed to target liver cells. For example, certain polymers or lipids can be modified with ligands that bind to receptors on the surface of liver cells, allowing for specific uptake of the nucleic acid complex. An example of such a ligand is asialoglycoprotein, which binds to the asialoglycoprotein receptor on hepatocytes. Another strategy for liver-targeted transfection is the use of viral vectors that have a natural tropism for liver cells. For example, adeno-associated virus (AAV) vectors have been shown to efficiently transduce liver cells in vivo, and can be engineered to express a specific gene of interest. Efficient and targeted liver transfection can be a challenge, as the liver is a complex organ with multiple cell types and functions. Altogen Liver In Vivo Transfection Reagent offers advanced delivery system that optimized for the specificity and efficiency of liver-targeted delivery.

Volume Options:

  • 0.5 ml – 10 injections (Catalog #5060)
  • 1.5 ml – 30 injections (Catalog #5061)
  • 8.0 ml – 160 injections (Catalog #5062)
  • 25 ml – 50 rat injections or 500 mouse injections (Catalog #5063)

Additional information

Weight 1 lbs
Dimensions 4 × 3 × 0.2 in
Kit Size (Volume)

0.5 ml – 10 injections (Catalog #5060), 1.5 ml – 30 injections (Catalog #5061), 8.0 ml – 160 injections (Catalog #5062), 25 ml – 50 rat injections or 500 mouse injections (Catalog #5063)