Lung In Vivo Transfection Kit

$495.00$11,100.00

Kit Size VolumePriceAdd to Cart
0.5 ml - 10 injections (Catalog #5080)$495.00
1.5 ml - 30 injections (Catalog #5081)$995.00
8.0 ml - 160 injections (Catalog #5082)$5,900.00
25 ml - 50 rat injections or 500 mouse injections (Catalog #5083)$11,100.00
SKU: LUNGIV Category:

Description

Product Availability: In Stock (FedEx delivery within 2 business days).

Ordering: To place an order please use Add to Cart button (account not required).


Lung In Vivo Transfection Reagent
Negatively charged small molecule, proteins, si/shRNA, mRNA and plasmid DNA lung-targeted in vivo delivery reagent for animal laboratory research (mouse, rat)

Modes of administration:

  • Intranasal
  • Inhalation (nebulized or volatilized)

Lung-targeted In Vivo Transfection Kit

  • Nanoparticle liposome-based reagent
  • Intranasal or inhalation administration
  • Efficient delivery to the lung tissue and lung tumor cells
  • Efficient protein, small molecule, RNA, and DNA delivery into lungs
  • No detectable toxicity
  • Functionally validated in mice
  • Download Lung-targeted in vivo transfection protocol: [PDF] [Word]
  • Download safety data sheet: [PDF]
  • UPC/GTIN/EAN: 860002089796
  • Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

DATA

In Vivo Liver Transfection Reagent Liver Hepatocarcinoma Tumors

Figure 1. Systemic administration (i.v.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Figure 2. Intratumoral administration (i.t.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Animal model is NOD/SCID xenograft mouse bearing flank tumor of human origin (liver cancer cell line). Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Liver-Targeted-Transfection-Altogen-Catalog-5062-4 Liver-Targeted-Transfection-Altogen-Catalog-5062-1 Liver-Targeted-Transfection-Altogen-Catalog-5062-2 Liver-Targeted-Transfection-Altogen-Catalog-5062-3

Figure 3. Systemic administration (i.v.) of Liver In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

Selected Liver InVivo Transfection Reagent citation references:

  • Molecular Therapy Nucleic Acids. 2017. Reverse Expression of Aging-Associated Molecules through Transfection … Kim H. et al [PDF]
  • PLoS Pathog. 2014 10(10) Exosomes from hepatitis C infected patients transmit HCV … Bukong et al [PDF]
  • Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
  • Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
  • J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]
  • J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]

Altogen Research Services:

Altogen Labs provides GLP compliant contract research services for IND applications and drug development. Our laboratory research services includes: Xenograft services (over 90 in-house validated xenograft models), development of stable cell lines (RNAi and protein production), ELISA assay development, cell-based and tissue targeted expression studies, safety pharmacology and toxicology assays, and many other preclinical research studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml – 10 injections (Catalog #5080)
  • 1.5 ml – 30 injections (Catalog #5081)
  • 8.0 ml – 160 injections (Catalog #5082)
  • 25 ml – 50 rat injections or 500 mouse injections (Catalog #5083)

Additional information

Weight 1 lbs
Kit Size (Volume)

0.5 ml – 10 injections (Catalog #5080), 1.5 ml – 30 injections (Catalog #5081), 8.0 ml – 160 injections (Catalog #5082), 25 ml – 50 rat injections or 500 mouse injections (Catalog #5083)