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Transfection Reagent for BxPC-3 Cells (Pancreatic Cancer Cells, CRL-1687)
- Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Kit includes Transfection Enhancer reagent
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Work in the presence of serum
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Download PowerPoint presentation for BxPC3 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 92% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems BxPC3 Transfection Protocol: [PDF]
Download MSDS: [PDF]
BxPC-3 Cell Line:
One of the difficult cancer types to diagnose, pancreatic cancer also has some of the lowest 5-year survival rates. According to an article published in the Journal of General Physiology in the March of 2009, Cystic Fibrosis Transmembrane Conductance Receptors (CFTR) provide a pathway for the transport of bicarbonate ions across the apical membrane of the pancreas. Malfunctioning of CFTR has been linked to cancers that affect pancreatic exocrine cells. Preclinical studies of pancreatic cancer, one of the deadliest forms of cancer, can help researchers identify novel therapies and progressive treatments, improving the survival rates for patients diagnosed with this fatal disease. BxPC-3 is a pancreatic cancer cell line that is tumorigenic and a reliable host for in vitro transfection studies. The BxPC-3 cell line was initially established using pancreatic tissue derived from a sample of a 61-year-old female patient with adenocarcinoma. These cells have been tested positive for CFTR and fail to express the cystic fibrosis transmembrane conductance regulator (CFTR). BxPC-3 cells possess an epithelial cellular morphology, as well as make a useful transfection in vitro model. Altogen Biosystems provides the lipid-based BxPC-3 transfection reagent kit that has successfully aided the establishment of stable BxPC-3 pancreatic cancer cell lines and is a suitable host for studying mammalian embryonic tissue.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the BxPC-3 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Figure 2. Protein expression of Cyclophilin B in BxPC-3 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into BxPC-3 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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