KG-1 Electroporation Kit (Leukemia Cells, CCL-246)

$396.00$799.00

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SKU: KG1E Category:

Description

Product Availability: In Stock (FedEx delivery within 2 business days).

Ordering: To place an order please use Add to Cart button (account not required).

Electroporation Kit for KG-1 Cells (Myelogenous Leukemia Cells, CCL-246)

• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems

Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette

Electroporation Efficiency:
Electroporation kit provides 70-85% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.

Electroporation Protocol and MSDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download MSDS: [PDF]

KG-1 Cell Line:
The KG-1 cell line was established from the bone marrow of a 59-year-old Caucasian male patient who suffered from acute myelogenous leukemia (cancer of the blood or bone marrow). These cells exhibit myeloblast cell morphology, and express the HLA A30, A31, B35, and Cw4 antigens. KG-1 cells are EBNA negative, and respond well to colony stimulating factor (CSF). These cells are used for cancer biomedical research, especially related to bone marrow and leukemia.

Mutations:

PTGDR 5729 37 14 52741530 52741530 Nonsense_Mutation SNP C T
FHOD3 80206 37 18 34320643 34320643 Missense_Mutation SNP G A
DUSP8 1850 37 11 1586951 1586951 Missense_Mutation SNP C G
GPR153 387509 37 1 6314891 6314891 Missense_Mutation SNP A T
VPS13D 55187 37 1 12516153 12516153 Missense_Mutation SNP C T
LRRC38 126755 37 1 13839711 13839711 Silent SNP C T
PRDM2 7799 37 1 14107576 14107576 Missense_Mutation SNP C T
NCDN 23154 37 1 36026283 36026283 Silent SNP C T
ADPRHL2 54936 37 1 36557618 36557618 Silent SNP C T
CYP4A22 284541 37 1 47607788 47607788 Missense_Mutation SNP T G
JUN 3725 37 1 59248138 59248138 Missense_Mutation SNP T G
GBP1 2633 37 1 89528786 89528786 Silent SNP C G
NOTCH2 4853 37 1 120466578 120466578 Missense_Mutation SNP T C

 

Data:
KG1 Electroporation Kit

 

Altogen Biosystems:

Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits. Advanced formulation of reagents and optimized electroporation protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about electroporation technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP compliant contract research studies for preclinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 4 ml (Catalog #6575)
  • 30 ml (Catalog #6576)

Additional information

Weight 1 lbs
Kit Size (Volume)

4 ml (Catalog #6575), 30 ml (Catalog #6576)