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Transfection Reagent for LS-174T Cells (Colon Adenocarcinoma Cell, CL-188)
- Proprietary cationic lipids formulation
Kit includes Transfection Enhancer reagent
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Work in the presence of serum
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Download LS174 CRISPR/Cas9 transfection protocol: [PDF]
- Download PowerPoint presentation for LS174T cells transfection kit: [PPT]
- UPC/GTIN/EAN: 860002089786
Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems
Reagent exhibits at least 92% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Enhanced transfection kit designed for superior efficiency in the LS174T cell line, a human colorectal adenocarcinoma cels.
Transfection Protocol and SDS:
Download Altogen Biosystems LS174T Transfection Protocol: [PDF]
Download SDS: [PDF]
LS-174T Cell Line:
LS174T is a human colorectal adenocarcinoma cell line that was originally isolated from a metastatic lesion in a patient with colorectal cancer. These cells have been widely used as a model system for studying various aspects of colorectal cancer biology, including the molecular mechanisms underlying tumor development and progression, and the identification of potential therapeutic targets for the treatment of colorectal cancer. LS174T cells have several properties that make them useful for research. Firstly, they are relatively easy to culture in the laboratory and can be grown in large quantities. Secondly, they express markers of colorectal cancer, such as carcinoembryonic antigen (CEA) and cytokeratin, and exhibit several features characteristic of colorectal cancer, including uncontrolled proliferation, invasion, and metastasis. Thirdly, they respond to various stimuli, such as cytokines and growth factors, making them a useful model system for studying the effects of these factors on colorectal cancer cell growth and survival. Research using LS174T cells has contributed significantly to our understanding of colorectal cancer biology. For example, LS174T cells have been used to study the role of various signaling pathways, such as the Wnt/β-catenin pathway, in the development and progression of colorectal cancer. Additionally, LS174T cells have been used to screen for potential anticancer agents, including chemotherapy drugs and targeted therapies.
Colorectal cancer cell lines represent accurate molecular models of primary carcinomas and have proven to be potent tools in preclinical colon cancer research. The LS 174T epithelial cell line was isolated from a 58-year-old Caucasian female with colorectal adenocarcinoma and produces high amounts of carcinoembryonic antigen (CEA). In addition to being positive for the expression of different oncogenes, such as c-myc, N-myc, H-ras, N-ras, Myb, and fos, the LS 174T cell line possesses abundant microvilli and vacuoles. LS 174T cells are positive for mRNA expression; however, they are negative for p53 antigen expression. This cell line is commonly used for oncological research as these cells produce vast amounts of carcinoembryonic antigen, a tumor marker. Altogen Biosystems supplies multiple in vitro transfection kits, including the cationic lipids formulation LS 174T transfection reagent kit for human colon cancer cells. The LS 174T reagent is compatible with both standard and reverse transfection methods, yielding very high transfection efficiency, as indicated by qRT-PCR.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the LS174T cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Figure 2. Protein expression of Cyclophilin B in LS-174T cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into LS-174T cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.
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