LoVo Transfection Reagent (Colon Adenocarcinoma)

$290.00$1,525.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #6840)$290.00
1.5 ml (Catalog #6841)$575.00
1.5 ml CRISPR (Catalog #2167)$775.00
8.0 ml (Catalog #7057)$1,525.00
SKU: LOVO Category:

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

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Transfection Reagent for LoVo Cells (Colon Adenocarcinoma Cells, CCL-229)

  • Two component formulation enhances lipid mediated transfection efficiency
  • Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
  • Reproducible transfection results
  • Works well for standard reverse transfection and high-throughput applications
  • Download in vitro LoVo transfection protocol: [PDF]
  • Download LoVo CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for LoVo cells transfection kit: [PPT]
  • Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 94% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems LoVo Transfection Protocol: [PDF]

Download MSDS: [PDF]

LoVo Cell Line:

Colorectal cancer is the third most commonly detected cancer in the United States, reportedly accounting for nearly 50,000 fatalities annually, as per the American Cancer Society (ACS). The LoVo epithelial cell line was isolated in 1971 from a metastatic tumor of a 56-year-old Caucasian male with colorectal adenocarcinoma. LoVo cells express multiple oncogenes, including N-ras, H-ras, Myb, c-myc, K-ras, sis and fos genes. Also, CC-3 and CC-4 tumor-specific nuclear matrix proteins are expressed. However, the cells are negative for expression of colon antigen 3 and CSAp (CSAp-). The LoVo cell line presents an aneuploid karyotype with a modal number of 49 and a doubling time of approximately 37 hours. A broad range of LoVo cells shows an extra B chromosome as well as altered A2. The LoVo cell line is a suitable host for studying various types of colon cancer. Altogen Biosystems provides highly efficient transfection reagent kits for the LoVo cell line.

Mutations:

KRAS 3845 37 12 25398281 25398281 Missense_Mutation SNP C T
FBXW7 55294 37 4 153247289 153247289 Missense_Mutation SNP G A
GRM7 2917 37 3 7620310 7620310 Nonsense_Mutation SNP C T
PIK3CB 5291 37 3 138374293 138374293 Missense_Mutation SNP C T
DCDC1 341019 37 11 30953328 30953328 De_novo_Start_OutOfFrame SNP G A
TRPV3 162514 37 17 3431412 3431412 Silent SNP C T
KIAA1644 85352 37 22 44681447 44681447 Missense_Mutation SNP G A
DTX3L 151636 37 3 122284753 122284753 Missense_Mutation SNP G A
LIX1 167410 37 5 96430694 96430694 Missense_Mutation SNP G A
ANKHD1 54882 37 5 139886500 139886500 Silent SNP G A
SLC17A2 10246 37 6 25921500 25921500 Silent SNP C A
HIST1H4G 8369 37 6 26247163 26247163 Missense_Mutation SNP C T
CHRNA2 1135 37 8 27320707 27320707 Missense_Mutation SNP T A

Data:

LoVo Cells Transfection Kit

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into LoVo cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

LoVo-cells-transfection-protocol

Figure 2. Protein expression of Lamin A in LoVo cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into LoVo cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Selected LoVo transfection reagent citations:

  • Carcinogenesis. 2014. A mechanistic study of the proapoptotic effect of tolfenamic acid … Jeong et al [PDF]
  • Diabetologia. 2012 55(7):2069-79. The p47phox- and NADPH oxidase organiser 1 … Youn et al [PDF]

Altogen Biosystems:
Altogen Biosystems transfection and electroporation products for life sciences and cancer research. Company developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits).  Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6840)
  • 1.5 ml (Catalog #6841)
  • 1.5 ml CRISPR (Catalog #2167)
  • 8.0 ml (Catalog #7057)

Additional information

Weight 1 lbs
Kit Size (Volume)

0.5 ml (Catalog #6840), 1.5 ml (Catalog #6841), 1.5 ml CRISPR (Catalog #2167), 8.0 ml (Catalog #7057)