LoVo Transfection Reagent (Colon Adenocarcinoma)

$390.00$2,025.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #6840)$390.00
1.5 ml (Catalog #6841)$775.00
1.5 ml CRISPR (Catalog #2167)$775.00
8.0 ml (Catalog #7057)$2,025.00
SKU: LOVO Category:

Description

Product Availability: In Stock (FedEx delivery within 2 business days).

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Transfection Reagent for LoVo Cells (Colon Adenocarcinoma Cells, CCL-229)

  • Two component formulation enhances lipid mediated transfection efficiency
  • Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
  • Reproducible transfection results
  • Works well for standard reverse transfection and high-throughput applications
  • Download in vitro LoVo transfection protocol: [PDF]
  • Download LoVo CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for LoVo cells transfection kit: [PPT]
  • UPC/GTIN/EAN: 860002089787
  • Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 94% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems LoVo Transfection Protocol: [PDF]

Download MSDS: [PDF]

LoVo Cell Line:

Colorectal cancer is the third most commonly detected cancer in the United States, reportedly accounting for nearly 50,000 fatalities annually, as per the American Cancer Society (ACS). The LoVo epithelial cell line was isolated in 1971 from a metastatic tumor of a 56-year-old Caucasian male with colorectal adenocarcinoma. LoVo cells express multiple oncogenes, including N-ras, H-ras, Myb, c-myc, K-ras, sis and fos genes. Also, CC-3 and CC-4 tumor-specific nuclear matrix proteins are expressed. However, the cells are negative for expression of colon antigen 3 and CSAp (CSAp-). The LoVo cell line presents an aneuploid karyotype with a modal number of 49 and a doubling time of approximately 37 hours. A broad range of LoVo cells shows an extra B chromosome as well as altered A2. The LoVo cell line is a suitable host for studying various types of colon cancer. Altogen Biosystems provides highly efficient transfection reagent kits for the LoVo cell line.

Mutations:

KRAS384537122539828125398281Missense_MutationSNPCT
FBXW755294374153247289153247289Missense_MutationSNPGA
GRM7291737376203107620310Nonsense_MutationSNPCT
PIK3CB5291373138374293138374293Missense_MutationSNPCT
DCDC134101937113095332830953328De_novo_Start_OutOfFrameSNPGA
TRPV3162514371734314123431412SilentSNPCT
KIAA16448535237224468144744681447Missense_MutationSNPGA
DTX3L151636373122284753122284753Missense_MutationSNPGA
LIX11674103759643069496430694Missense_MutationSNPGA
ANKHD154882375139886500139886500SilentSNPGA
SLC17A2102463762592150025921500SilentSNPCA
HIST1H4G83693762624716326247163Missense_MutationSNPCT
CHRNA211353782732070727320707Missense_MutationSNPTA

Data:

LoVo Cells Transfection Kit

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into LoVo cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

LoVo-cells-transfection-protocol

Figure 2. Protein expression of Lamin A in LoVo cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into LoVo cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Selected LoVo transfection reagent citations:

  • Carcinogenesis. 2014. A mechanistic study of the proapoptotic effect of tolfenamic acid … Jeong et al [PDF]
  • Diabetologia. 2012 55(7):2069-79. The p47phox- and NADPH oxidase organiser 1 … Youn et al [PDF]

Altogen Biosystems:
Altogen Biosystems transfection and electroporation products for life sciences and cancer research. Company developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits).  Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6840)
  • 1.5 ml (Catalog #6841)
  • 1.5 ml CRISPR (Catalog #2167)
  • 8.0 ml (Catalog #7057)

Additional information

Weight1 lbs
Dimensions4 × 3 × 0.2 in
Kit Size (Volume)

0.5 ml (Catalog #6840), 1.5 ml (Catalog #6841), 1.5 ml CRISPR (Catalog #2167), 8.0 ml (Catalog #7057)