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Transfection Reagent for IRR-MRC5 Cells (Irradiated Fibroblast Cells, 55-X)
- Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Work in the presence of serum
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Download PowerPoint presentation for IRR-MRC5 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 73% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems IRR-MRC5 Transfection Protocol: [PDF]
Download MSDS: [PDF]
IRR-MRC5 Cell Line:
The IRR-MRC-5 cell line was established from the lung tissue of a 14-week-old Caucasian male fetus. The main purpose of these cells is to be used to feed and support the growth of other cells. The “IRR” indicates that they have been irradiated, and therefore will not replicate or divide like other cells. IRR-MRC-5 cells exhibit fibroblast morphology, and on their own, could be useful for studying human embryonic lung tissue.
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into IRR-MRC5 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in IRR-MRC5 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into IRR-MRC5 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
- 0.5 ml (Catalog #6833)
- 1.5 ml (Catalog #6834)
- 8.0 ml (Catalog #7050)