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Transfection Reagent for HeLa-S3 Cells (Cervical Adenocarcinoma, CCL-2.2)
- Proprietary cationic lipids formulation
- High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
- Effective and robust intracellular delivery
- Kit includes Transfection Enhancer reagent
- Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
- Work in the presence of serum
- A proven reagent for establishing stable cell lines
- Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Download HeLaS3 CRISPR/Cas9 transfection protocol: [PDF]
- Download PowerPoint presentation for HeLa-S3 cells transfection kit: [PPT]
- Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 94% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems Hela-S3 Transfection Protocol: [PDF]
Download MSDS: [PDF]
HeLa-S3 Cell Line:
The HeLa S3 cell line is a derivative of the immortal HeLa cell line and is also considered immortal, sharing many similar properties with the HeLa cell line, as a result. The difference between the famous HeLa cell line and the HeLa S3 cell line is that S3 represents the third clone isolated and propagated from the original HeLa culture. The letter S was used in honor of Dr. Florence Rena Sabin, though this cell line originated in the lab of Dr. Theodore Puck. HeLa S3 was established in 1955 and has been used for studies in cloning of mammal cells. It also has similar properties to the famous HeLa cell line, named after Henrietta Lacks, from which it was derived. The HeLa cell line was established using cervical cancer cells taken in 1951 from Henrietta Lacks, a patient who eventually died of her cancer later that year. The HeLa cell line considered an “immortal” cell line and had been used in a wide variety of scientific research. These cells have received a lot of media attention, due to the controversy surrounding patient’s rights. The HeLa cell line was in fact the first human cell line to be able to establish itself and be successful in a cell culture. In addition to being an indispensable tool in conducting a wide variety of specialized biomedical applications, the HeLa S3 is beneficial for mammalian cloning research. The HeLa S3 transfection reagent kit for cervical adenocarcinoma offered by Altogen Biosystems, among many other transfection products, yields high transfection efficiency of siRNA delivery. Besides, it produces high-level recombinant protein expression with minimal disruption of cell function and has low cytotoxicity.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the Hela-S3 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Figure 2. Protein expression of Cyclophilin B in HeLa-S3 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into HeLa-S3 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
- 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
- Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]
- Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]
- Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]
- 2010 16(11):2108-19. RNase L releases a small RNA from HCV RNA that refolds … Malathi et al [PDF]
- 2012 55(7):2069-79. The p47phox- and NADPH oxidase organiser 1 … Youn et al [PDF]
- British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]
- 2014 63(2):353-61. Tissue transglutaminase contributes to … Liu et al [PDF]
- Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]
- 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
Altogen Biosystems transfection and electroporation products for life sciences and cancer research. Company developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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