HL-60 Electroporation Kit (Plasmacytoma, CCL-240)

$285.00$592.00

Kit Size VolumePriceAdd to Cart
4 ml (Catalog #1751)$285.00
30 ml (Catalog #1752)$592.00
SKU: HL60 Category:

Description

NOTE: To place order using Credit Card, P-Card, or Purchase Order – please choose kit size above and click “Add to cart” button. You can place order online without creating an account.

Electroporation Kit for HL-60 Cells (Plasmacytoma Cells, CCL-240)

 • Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems

Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette

Electroporation Efficiency:
Electroporation kit provides 65-80% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.

Electroporation Protocol and MSDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download MSDS: [PDF]

HL-60 Cell Line:
The tumorigenic HL-60 cell line was established using the peripheral blood tissue from a 36-year-old female patient who suffered from acute promyelocytic leukemia (APL). This cell line exhibits myeloblastic cell morphology and a promyeloblast cell type. These cells positively express the myc oncogene, and was derived by S.J. Collins, et all. HL-60 have been known to spontaneously differentiate, this can even be stimulated and controlled, to an extent, by a variety of compounds. This cell line is useful as a transfection host in scientific research.

Mutations:

GRID1289437108748414587484145Missense_MutationSNPCT
KLK6565337195147053751470537Missense_MutationSNPCT
SEMG2640737204385114743851147Missense_MutationSNPCT
PRDM166397637131027123102712Missense_MutationSNPAG
FUCA125173712417516124175161Missense_MutationSNPCG
AHDC1272453712787841227878412Missense_MutationSNPCT
TSSK3816293713282923632829236SilentSNPCT
CPT213763715367924253679242Missense_MutationSNPTC
CYP2J215733716036666260366662Missense_MutationSNPCA
CELSR21952371109793998109793998Missense_MutationSNPGA

 

Data:
HL60 Electroporation Kit

Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):

  • 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
  • Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]
  • Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]
  • Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]

 

Altogen Biosystems:

Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits. Advanced formulation of reagents and optimized electroporation protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about electroporation technology at Altogen’s Transfection Resource.

Altogen Research Services:

Altogen Labs provides GLP compliant contract research studies for preclinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 4 ml (Catalog #1751)
  • 30 ml (Catalog #1752)

Additional information

Weight1 lbs
Kit Size (Volume)

4 ml (Catalog #1751), 30 ml (Catalog #1752)