NCI-H1975 Transfection Kit (Lung Carcinoma)

$309.00$1,678.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #6854)$309.00
1.5 ml (Catalog #6855)$626.00
1.5 ml CRISPR (Catalog #2177)$826.00
8.0 ml (Catalog #7067)$1,678.00
SKU: NCIH1975 Category:

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

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Transfection Reagent for NCI-H1975 Cells (Lung Adenocarcinoma Cells, CRL-5908)

Transfection Efficiency:

Reagent exhibits at least 94% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems NCI-H1975 Transfection Protocol: [PDF]

Download MSDS: [PDF]

NCI-H1975 Cell Line:

Although lung cancer therapeutics have evolved dramatically within last two decades, lung cancer is considered the primary cause of cancer deaths globally in both men and women. Non-small-cell lung cancers comprise approximately 80% of all lung cancer incidences and include adenocarcinomas, squamous cell carcinomas as well as large cell carcinomas. Non-small cell lung cancer is frequently diagnosed at advanced stages, with the average 5-year survival rate of only 16%. New approaches in lung cancer therapy are crucial in treating advanced-stage patients. The NCI-H1975 epithelial cell line was isolated in 1988 from lung cells taken from a non-smoker female with adenocarcinoma. NCI-H1975 is useful in investigative and analytical studies of new lung cancer drugs. Altogen Biosystems provides high-efficiency transfection reagent kits for the NCI-H1975 cell line.

Cell line mutations:

TP537157371775771207577120Missense_MutationSNPCT
PIK3CA5290373178917478178917478Splice_SiteSNPGA
EGFR19563775525951555259515Missense_MutationSNPTG
KLK81120237195149937751499377Missense_MutationSNPCT
OR2T27403239371248813821248813821Missense_MutationSNPCT
CDKN2A10293792197115321971153Nonsense_MutationSNPCA
TTN7273372179411904179411904Missense_MutationSNPGA
ERN21059537162371198623711986Missense_MutationSNPGC
EMR38465837191474891814748918Splice_SiteSNPGA
MAP24133372210560646210560646Missense_MutationSNPGA
COL12A113033767584449975844499Missense_MutationSNPCT

Data:

NCI-H1975 Transfection Reagent

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the NCI-H1975 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

NCI-H1975-cells-transfection-protocol

Figure 2. Protein expression of Cyclophilin B in NCI-H1975 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into NCI-H1975 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems provides optimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient cellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP compliant contract research studies for preclinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6854)
  • 1.5 ml (Catalog #6855)
  • 1.5 ml CRISPR (Catalog #2177)
  • 8.0 ml (Catalog #7067)

Additional information

Weight1 lbs
Kit Size (Volume)

0.5 ml (Catalog #6854), 1.5 ml (Catalog #6855), 1.5 ml CRISPR (Catalog #2177), 8.0 ml (Catalog #7067)