NCI-H1975 Transfection Kit (Lung Carcinoma)

$309.00$1,678.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #6854)$309.00
1.5 ml (Catalog #6855)$626.00
1.5 ml CRISPR (Catalog #2177)$826.00
8.0 ml (Catalog #7067)$1,678.00
SKU: NCIH1975 Category:

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

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Transfection Reagent for NCI-H1975 Cells (Lung Adenocarcinoma Cells, CRL-5908)

Transfection Efficiency:

Reagent exhibits at least 94% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems NCI-H1975 Transfection Protocol: [PDF]

Download MSDS: [PDF]

NCI-H1975 Cell Line:

Although lung cancer therapeutics have evolved dramatically within last two decades, lung cancer is considered the primary cause of cancer deaths globally in both men and women. Non-small-cell lung cancers comprise approximately 80% of all lung cancer incidences and include adenocarcinomas, squamous cell carcinomas as well as large cell carcinomas. Non-small cell lung cancer is frequently diagnosed at advanced stages, with the average 5-year survival rate of only 16%. New approaches in lung cancer therapy are crucial in treating advanced-stage patients. The NCI-H1975 epithelial cell line was isolated in 1988 from lung cells taken from a non-smoker female with adenocarcinoma. NCI-H1975 is useful in investigative and analytical studies of new lung cancer drugs. Altogen Biosystems provides high-efficiency transfection reagent kits for the NCI-H1975 cell line.

Cell line mutations:

TP53 7157 37 17 7577120 7577120 Missense_Mutation SNP C T
PIK3CA 5290 37 3 178917478 178917478 Splice_Site SNP G A
EGFR 1956 37 7 55259515 55259515 Missense_Mutation SNP T G
KLK8 11202 37 19 51499377 51499377 Missense_Mutation SNP C T
OR2T27 403239 37 1 248813821 248813821 Missense_Mutation SNP C T
CDKN2A 1029 37 9 21971153 21971153 Nonsense_Mutation SNP C A
TTN 7273 37 2 179411904 179411904 Missense_Mutation SNP G A
ERN2 10595 37 16 23711986 23711986 Missense_Mutation SNP G C
EMR3 84658 37 19 14748918 14748918 Splice_Site SNP G A
MAP2 4133 37 2 210560646 210560646 Missense_Mutation SNP G A
COL12A1 1303 37 6 75844499 75844499 Missense_Mutation SNP C T

Data:

NCI-H1975 Transfection Reagent

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the NCI-H1975 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

NCI-H1975-cells-transfection-protocol

Figure 2. Protein expression of Cyclophilin B in NCI-H1975 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into NCI-H1975 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems provides optimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient cellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP compliant contract research studies for preclinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6854)
  • 1.5 ml (Catalog #6855)
  • 1.5 ml CRISPR (Catalog #2177)
  • 8.0 ml (Catalog #7067)

Additional information

Weight 1 lbs
Kit Size (Volume)

0.5 ml (Catalog #6854), 1.5 ml (Catalog #6855), 1.5 ml CRISPR (Catalog #2177), 8.0 ml (Catalog #7067)