CCRF-CEM Electroporation Kit (CML Leukemia Cells)

$280.00$590.00

Kit Size VolumePriceAdd to Cart
4 ml (Catalog #6559)$280.00
30 ml (Catalog #6560)$590.00
SKU: CCRF Category:

Description

NOTE: To place order using Credit Card, P-Card, or Purchase Order – please choose kit size above and click “Add to cart” button. You can place order online without creating an account.

Electroporation Kit for CCRF-CEM Cells (CML Leukemia Cells, CCL-119)

  • Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
  • Preoptimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
  • Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)
  • Certified RNAse- and DNAse-free electroporation buffer
  •  Effective electroporation delivery and high cell viability provides reproducible electroporation results
  • High cell viability and superior cell recovery rates
  • Electroporation protocols are provided with with the purchase of the Electroporation Kit
  • UPC/GTIN/EAN: 860002089748
  • Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette

Electroporation Efficiency:
Electroporation kit provides 80-95% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.

Electroporation Protocol and MSDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download MSDS: [PDF]

CCRF-CEM Cell Line:
The CCRF-CEM cell line was established in 1964 from the peripheral blood of a 4-year-old Caucasian female who suffered from acute lymphoblastic leukemia. These tumorigenic cells exhibit lymphoblast morphology and a T lymphoblast cell type. CCRF-CEM cells positively express CD3 – CD7 antigens, except for CD6. These cells could be useful for transfection experiments to study leukemia.

Mutations:

TP53 7157 17 7577538 7577538 Missense_Mutation SNP C T
FBXW7 55294 4 153249385 153249385 Missense_Mutation SNP G A
GRIN2A 2903 16 9858202 9858202 Missense_Mutation SNP G A
SLC26A5 375611 7 103061308 103061308 Frame_Shift_Del DEL T
CTCF 10664 16 67654642 67654642 Missense_Mutation SNP C T
BRINP1 1620 9 121929543 121929543 Missense_Mutation SNP C T
NOTCH2NL 388677 1 145273244 145273244 Missense_Mutation SNP G A
TLR5 7100 1 223284999 223284999 Missense_Mutation SNP G A
KRT14 3861 17 39740077 39740077 Missense_Mutation SNP G A
EHMT1 79813 9 140638532 140638532 Missense_Mutation SNP G A
USP15 9958 12 62786091 62786091 Frame_Shift_Del DEL A
PALB2 79728 16 23646981 23646981 Frame_Shift_Del DEL T
PPFIBP1 8496 12 27841317 27841317 Silent SNP G A
FLT3 2322 13 28608087 28608087 Missense_Mutation SNP C T
CLMN 79789 14 95670573 95670573 Silent SNP C T
DTNA 1837 18 32438277 32438277 Missense_Mutation SNP C T
CD93 22918 20 23065010 23065010 Missense_Mutation SNP C T
MB21D2 151963 3 192516769 192516769 Silent SNP G A
HIST1H4G 8369 6 26247163 26247163 Missense_Mutation SNP C T
EZH2 2146 7 148515169 148515169 Missense_Mutation SNP C T

 

Data:
CCRF-CEM Electroporation Kit

Altogen Biosystems:

Altogen Biosystems is a life sciences company that manufactures over 120 cell type specific and optimized transfection reagents, electroporation buffers, and targeted in vivo delivery kits. Advanced formulation of reagents and optimized electroporation protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about electroporation technology at Altogen’s Transfection Resource.

Altogen Laboratories Contract Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 4 ml (Catalog #6559)
  • 30 ml (Catalog #6560)

Additional information

Weight 1 lbs
Kit Size (Volume)

4 ml (Catalog #6559), 30 ml (Catalog #6560)