CCRF-CEM Electroporation Kit (CML Leukemia Cells)

$280.00$590.00

Kit Size VolumePriceAdd to Cart
4 ml (Catalog #6559)$280.00
30 ml (Catalog #6560)$590.00
SKU: CCRF Category:

Description

NOTE: To place order using Credit Card, P-Card, or Purchase Order – please choose kit size above and click “Add to cart” button. You can place order online without creating an account.

Electroporation Kit for CCRF-CEM Cells (CML Leukemia Cells, CCL-119)

  • Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
  • Preoptimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
  • Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)
  • Certified RNAse- and DNAse-free electroporation buffer
  •  Effective electroporation delivery and high cell viability provides reproducible electroporation results
  • High cell viability and superior cell recovery rates
  • Electroporation protocols are provided with with the purchase of the Electroporation Kit
  • Developed and manufactured by Altogen Biosystems

Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette

Electroporation Efficiency:
Electroporation kit provides 80-95% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.

Electroporation Protocol and MSDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download MSDS: [PDF]

CCRF-CEM Cell Line:
The CCRF-CEM cell line was established in 1964 from the peripheral blood of a 4-year-old Caucasian female who suffered from acute lymphoblastic leukemia. These tumorigenic cells exhibit lymphoblast morphology and a T lymphoblast cell type. CCRF-CEM cells positively express CD3 – CD7 antigens, except for CD6. These cells could be useful for transfection experiments to study leukemia.

Mutations:

TP5371571775775387577538Missense_MutationSNPCT
FBXW7552944153249385153249385Missense_MutationSNPGA
GRIN2A29031698582029858202Missense_MutationSNPGA
SLC26A53756117103061308103061308Frame_Shift_DelDELT
CTCF10664166765464267654642Missense_MutationSNPCT
BRINP116209121929543121929543Missense_MutationSNPCT
NOTCH2NL3886771145273244145273244Missense_MutationSNPGA
TLR571001223284999223284999Missense_MutationSNPGA
KRT143861173974007739740077Missense_MutationSNPGA
EHMT1798139140638532140638532Missense_MutationSNPGA
USP159958126278609162786091Frame_Shift_DelDELA
PALB279728162364698123646981Frame_Shift_DelDELT
PPFIBP18496122784131727841317SilentSNPGA
FLT32322132860808728608087Missense_MutationSNPCT
CLMN79789149567057395670573SilentSNPCT
DTNA1837183243827732438277Missense_MutationSNPCT
CD9322918202306501023065010Missense_MutationSNPCT
MB21D21519633192516769192516769SilentSNPGA
HIST1H4G836962624716326247163Missense_MutationSNPCT
EZH221467148515169148515169Missense_MutationSNPCT

 

Data:
CCRF-CEM Electroporation Kit

Altogen Biosystems:

Altogen Biosystems is a life sciences company that manufactures over 120 cell type specific and optimized transfection reagents, electroporation buffers, and targeted in vivo delivery kits. Advanced formulation of reagents and optimized electroporation protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about electroporation technology at Altogen’s Transfection Resource.

Altogen Laboratories Contract Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 4 ml (Catalog #6559)
  • 30 ml (Catalog #6560)

Additional information

Weight1 lbs
Kit Size (Volume)

4 ml (Catalog #6559), 30 ml (Catalog #6560)