NTERA-2 Transfection Kit (Embryonal Carcinoma)

$294.00$1,549.00

Kit Size VolumePriceAdd to Cart
0.5 ml (Catalog #6875)$294.00
1.5 ml (Catalog #6876)$583.00
1.5 ml CRISPR (Catalog #2186)$783.00
8.0 ml (Catalog #7075)$1,549.00
SKU: NTERA2 Category:

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

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Transfection Reagent for NTERA-2 Cells (Embryonal Carcinoma Cells, CRL-1973)

  • Two component formulation enhances lipid mediated transfection efficiency
  • Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
  • Kit includes Transfection Enhancer reagent and recommended transfection protocol
  • Reproducible transfection results
  • Download in vitro NTERA2 transfection protocol: [PDF]
  • Download NTERA2 CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for NTERA2 cells transfection kit: [PPT]
  • Developed and manufactured by Altogen Biosystems
Transfection Efficiency:

Reagent exhibits at least 76% transfection efficiency of siRNA delivery. Transfection efficiency was determined by RT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems NTERA2 Transfection Protocol: [PDF]

Download MSDS: [PDF]

NTERA-2 Cell Line:

Embryonal carcinomas have proven to be invaluable models for studying the mechanisms that control differentiation of cells during embryogenesis in vitro. The NTERA-2 cell line is considered a well-characterized system of ectodermal differentiation. NTERA-2 cells can be used to study the early stages of human neurogenesis as they display developmental and biochemical properties similar to embryonic cells. The NTERA-2 cl.D1 cell line was established from the parental NTERA-2 cell line after a xenograft of a 22-year-old Caucasian male with malignant pluripotent embryonic carcinoma of the testis. This cell line has unique properties, making it useful for studying of certain types of testicular cancer. These cells exhibit high activity expression of N-myc oncogene. The cell line contains untreated cells with resistance to HCMV, and HIV-1. NTERA-2 cl.D1 exhibits an epithelial-like morphology, though there is more variety than in other cell lines due to the high differentiation of these cells. Altogen Biosystems manufactures lipid-based transfection kit optimized for NTERA-2 cells that can be utilized for both transient transfection and development of stable NTERA-2 cell lines and commonly used for life sciences laboratory research studies.

Cell line mutations:

DEGS2 123099 37 14 100615443 100615443 Silent SNP G A
NLRP8 126205 37 19 56490859 56490859 Silent SNP G A
GPC1 2817 37 2 241398519 241398519 Missense_Mutation SNP G A
TECPR1 25851 37 7 97857345 97857345 Missense_Mutation SNP C T
GPR153 387509 37 1 6314173 6314173 Missense_Mutation SNP C T
NBPF1 55672 37 1 16913645 16913645 Silent SNP G A
ZSCAN20 7579 37 1 33960037 33960037 Missense_Mutation SNP T C
ZMYM4 9202 37 1 35864555 35864555 Missense_Mutation SNP A T

Data:

NTERA2 Transfection Reagent

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into NTERA-2 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by RT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

NTERA2-cells-transfection-protocol

Figure 2. Protein expression of Lamin A in NTERA-2 cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into NTERA-2 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems is a life sciences company that offers cell type-specific and optimized transfection products, electroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient cellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for preclinical research, IND applications, and drug development. Biology CRO services include: xenograft models (90+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6875)
  • 1.5 ml (Catalog #6876)
  • 1.5 ml CRISPR (Catalog #2186)
  • 8.0 ml (Catalog #7075)

Additional information

Weight 1 lbs
Kit Size (Volume)

0.5 ml (Catalog #6875), 1.5 ml (Catalog #6876), 1.5 ml CRISPR (Catalog #2186), 8.0 ml (Catalog #7075)