Kidney In Vivo Transfection Kit

$695.00$9,350.00

Kit Size VolumePricePay with CC, PO, or PaypalAdd to Cart
0.5 ml - 10 injections (Catalog #5070)$695.00
1.5 ml - 30 injections (Catalog #5071)$1,395.00
8.0 ml - 160 injections (Catalog #5072)$3,885.00
25 ml - 50 rat injections or 500 mouse injections (Catalog #5073)$9,350.00
SKU: KIDIV Category:

Description

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Kidney In Vivo Transfection Reagent
siRNA and DNA kidney in vivo delivery reagent for animal research (mouse, rat)

Modes of administration:

  • Systemic intravenous (i.v.) injection
  • Direct intratumoral (i.t.) injection
  • Intraperitoneal (i.p.) injection

Kidney-targeted In Vivo Transfection Reagent

  • Nanoparticle-based liposomal reagent
  • Nanoparticle/liposome conjugated complexes are stable in serum for 24 hours
  • Efficient delivery to the kidney tissue (via systemic administration) and kidney tumors (via intratumoral administration)
  • Efficient small RNA (si/sh/miRNA), mRNA, and plasmid DNA delivery
  • Minimal toxicity
  • Functionally validated in mice and rats
  • Applicable for plasmid DNA/siRNA co-injection
  • Download Kidney-targeted in vivo transfection protocol: [PDF] [Word]
  • Download PowerPoint presentation for Kidney-targeted in vivo transfection kit: [PPT]
  • Download safety data sheet: [PDF]
  • Developed and manufactured by Altogen Biosystems

DATA

In Vivo Kidney Transfection Kit Kidney Tumors

Figure 1. Systemic administration (i.v.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=7).

In Vivo Kidney Transfection Reagent

Figure 2. Intratumoral administration (i.t.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Animal model is NOD/SCID xenograft mouse bearing flank tumor of human origin (kidney cancer cell line). Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=7).

Kidney-Targeted-Transfection-Altogen-Catalog-5072-4 Kidney-Targeted-Transfection-Altogen-Catalog-5072-1 Kidney-Targeted-Transfection-Altogen-Catalog-5072-2 Kidney-Targeted-Transfection-Altogen-Catalog-5072-3

Figure 3. Systemic administration (i.v.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

Kidney Transfection Reagent citation references:

  • The Journal of Pathology. 2015 235(5):731–744. BDNF repairs podocyte damage by microRNA-mediated … Min Li et al [PDF]
  •  Nature Communications. 2016. 7:12076. miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy. Badal et al [PDF]
  •  Journal of the American Society of Nephrology. 2016 Nov;27(11):3308-3319. Synaptopodin Limits TRPC6 Podocyte Surface Expression and Attenuates Proteinuria. Yu H et al [PDF]
  • British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]
  • Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]
  • J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]

Kidney transfection (delivery)

Kidney transfection is a laboratory technique used to deliver nucleic acids, such as DNA, RNA, or siRNA, into kidney cells and tissues to modify their gene expression or to study gene function. Kidney transfection is a valuable tool for studying gene function and for developing new therapies for genetic diseases or kidney-related disorders. However, the efficiency and specificity of delivery in vivo can be affected by various factors, including the choice of transfection reagent, the type of target cells, and the experimental conditions.

Altogen Labs (Contract Research Services):

Altogen Labs provides GLP compliant preclinical laboratory research services, including efficacy and safety studies (IND applications and drug development). Nonclinical CRO services includes: Xenograft models (over 90 in-house validated xenograft models), histopathology, gene expression, and in vivo imaging studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml – 10 injections (Catalog #5070)
  • 1.5 ml – 30 injections (Catalog #5071)
  • 8.0 ml – 160 injections (Catalog #5072)
  • 25 ml – 50 rat injections or 500 mouse injections (Catalog #5073)

Additional information

Weight 1 lbs
Dimensions 4 × 3 × 0.2 in
Kit Size (Volume)

0.5 ml – 10 injections (Catalog #5070), 1.5 ml – 30 injections (Catalog #5071), 8.0 ml – 160 injections (Catalog #5072), 25 ml – 50 rat injections or 500 mouse injections (Catalog #5073)