AltoFect Transfection Reagent


Kit Size VolumePricePay with CC, PO, or PaypalAdd to Cart
0.05 ml (Catalog #3000) - Free Sample (w/paid shipping or FedEx acct)$0.00
0.5 ml (Catalog #3001)$402.00
1.5 ml (Catalog #3002)$850.00
8.0 ml (Catalog #3003)$2,310.00
SKU: AFTR Category:


Purchase Orders: Click “Add to Cart” button to order, then email PO to

Product Availability: In Stock.

Transfection Reagent for Primary Cells and Difficult-to-Transfect Cell Lines

  • Two component formulation enhances liposome mediated transfection efficiency
  • Transfection protocol provided for transfection of si/miRNA, plasmid DNA, mRNA, and small proteins
  • AltoFect™ Transfection Kit includes Transfection Enhancer reagent and recommended transfection protocol
  • Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
  • Download AltoFect™ transfection protocol: [PDF]
  • UPC/GTIN/EAN: 860002089701
  • Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

Product Description:

AltoFect reagent is designed for hard-to-transfect cell lines and primary cell types. These cells are are difficult to introduce foreign genetic material into using traditional transfection methods (transfection is a laboratory technique used to introduce nucleic acids, such as DNA, RNA, or siRNA, into cells, allowing researchers to study gene expression, protein function, and cell signaling pathways). Some cells are hard-to-transfect because they have innate mechanisms that prevent the entry of foreign RNA and protein into cytoplasm and DNA into their nuclei. These cells are usually highly specialized and have complex structures that make it difficult for biomolecules to enter. Examples of hard-to-transfect cells include primary cells (cells taken directly from living organisms rather than being grown in a lab), stem cells, neurons, and immune cells (suspension cultures). AltoFect is a powerfull research tool to overcome the challenges of transfecting hard-to-transfect cells, allowing researchers to study the functions of genes and their roles in cellular processes.

Transfection Efficiency:

Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR and Western Blot analysis.

Transfection Protocol and SDS:

Download Altogen Biosystems AltoFect™ Transfection Protocol: [PDF]

Download SDS: [PDF]


AltoFect Transfection Reagent

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in primary neuronal culture transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended AltoFect transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

AltoFect Transfection Reagent

Figure 2. Protein expression of Cyclophilin B in primary neuronal cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into primary neuronal cells following AltoFect™ transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems manufacturers over 100 pre-optimized in vitro transfection kits for cancer cell lines and primary cells, elecroporation products, and  tissue-targeted in vivo delivery reagents for life science research. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit

AltoFect Transfection Kit

Volume Options:

  • 0.05 ml (Catalog #3000)
  • 0.5 ml (Catalog #3001)
  • 1.5 ml (Catalog #3002)
  • 8.0 ml (Catalog #3003)

Purchase Orders: Click “Add to Cart” button to order, then email PO to

Product Availability: In Stock.

Additional information

Weight 1 lbs
Dimensions 4 × 3 × 0.2 in
Kit Size (Volume)

0.05 ml (Catalog #3000) – Free Sample (w/paid shipping or FedEx acct), 0.5 ml (Catalog #3001), 1.5 ml (Catalog #3002), 8.0 ml (Catalog #3003)