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Transfection Reagent for Primary Cells and Difficult-to-Transfect Cell Lines
- Two component formulation enhances liposome mediated transfection efficiency
- Transfection protocol provided for transfection of si/miRNA, plasmid DNA, mRNA, and small proteins
- AltoFect Transfection Kit includes Transfection Enhancer reagent and recommended transfection protocol
- Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
- Download AltoFect™ transfection protocol: [PDF]
- Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR and Western Blot analysis.
Transfection Protocol and MSDS:
Download Altogen Biosystems AltoFect™ Transfection Protocol: [PDF]
Download MSDS: [PDF]
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in primary neuronal culture transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended AltoFect transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Figure 2. Protein expression of Cyclophilin B in primary neuronal cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into primary neuronal cells following AltoFect™ transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
About Altogen Biosystems:
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, electroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for preclinical research, IND applications, and drug development. Biology CRO services include: Over 60 xenograft models, development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
- 0.05 ml (Catalog #3000)
- 0.5 ml (Catalog #3001)
- 1.5 ml (Catalog #3002)
- 8.0 ml (Catalog #3003)