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Altogen™ Transfection Reagents and In Vivo Delivery Kits

Altogen Biosystems is a biotechnology company that develops and manufactures transfection kits for life sciences laboratory research, drug discovery, and development. Altogen® In Vivo transfection reagents provide effective and targeted delivery of biomolecules into target tissues. In vitro cell transfection kits are optimized for specific cancer cell lines and primary cell types. Efficient delivery of cargo molecules (DNA, RNA, proteins) is enabled by advanced reagent formulation and optimized transfection protocols. Altogen Biosystems develops transfection reagents by optimizing polymer-stabilized liposomes, applying polymer chemistry, and molecular and cell biology expertise.

Altogen Biosystems offers optimized transfection kits and electroporation products for 120+ cancer cell lines and primary cells. Tissue-targeted delivery is enabled by in vivo transfection reagents. Altogen’s transfection kits include both the Transfection Enhancer and the Complex Condenser for transfection in vitro (cancer cell lines) and in vivo (tissue targeted reagents for research in rodents). The following in vivo transfection kits were featured by Science magazine:

In Vivo Kidney-targeted Transfection Reagent – Targeted tissues: Kidney, Kidney Tumors
In Vivo Nanoparticle-based Transfection Reagent – Targeted tissues: Lung, Liver, Heart, Kidney, Tumors
In Vivo PEG-liposome Transfection Reagent – Targeted tissues: Spleen, Liver, Kidney, Tumors
In Vivo Pancreas-targeted Transfection Reagent – Targeted tissues: Pancreas, Pancreas Tumors
In Vivo Liver-targeted Transfection Kit – Targeted tissues: Liver, Liver Tumors

Transfection is the delivery of exogenous molecules into cultured cells and is a commonly used laboratory method to study gene function, modulation of gene expression, biochemical mapping, and protein production. Unfortunately, no single delivery method or transfection reagent can be applied to all types of cells due to cellular cytotoxicity. Transfection efficiencies vary dramatically and depend on the reagent, protocol, and cell type used.

Selected citations and references (ALTOGEN® In Vivo Transfection Kits used in the following publications):

  • Nature. 2008 454(7203):523. Innate immunity induced by composition-dependent… Saito et al [PDF]
  • Nature Medicine. 2016 22:1131. The long noncoding RNA defines an epigenetic… Wang et al [PDF]
  • Nature Biotechnology. 2011 29:341. Delivery of siRNA to the mouse brain… Alvarez-Erviti et al [PDF]
  • Nature Communications. 2016 miR-93 regulates Msk2-mediated chromatin … Badal et al [PDF]
  • J Cereb Blood Flow Metab. 2017 Inhibition of Src family kinases improves cognitive… Liu et al [PDF]
  • Am J Pathol. 2016 Ubiquitin-Conjugating enzyme 9 phosphorylation mechanism… Tomasi et al [PDF]
  • J Biol Chem. 2017 Protein phosphotyrosine phosphatase in calpain-dependent… Zhang et al [PDF]
  • J Mol Cell Cardiol. 2015 Netrin-1 abrogates ischemia reperfusion-induced… Siu et al [PDF]
  • Diabetologia. 2012 55(7):2069. The p47phox- and NADPH oxidase organiser… Youn et al [PDF]
  • Journal American Society of Nephrology. 2016 TRPC6 podocyte surface expression… Yu H et al [PDF]
  • Hypertension. 2014 63(2):353. Tissue transglutaminase contributes… Liu et al [PDF]
  • Circulation Research. 2010 Kruppel-like factor-4 transcriptionally regulates… Cowan et al [PDF]
  • Hypertension. 2012 59:158. Role of uncoupled endothelial nitric oxide synthase… Gao et al [PDF]
  • Cardiovascular Research. 2016 Modulators right ventricular apoptosis… Zungu-Edmondson et al [PDF]
  • J Proteome Res. 2012 Retinal proteome analysis mouse model of oxygen-induced… Kim et al [PDF]
  • J Transl Med. 2010 Prevention hyperglycemia-induced myocardial apoptosis… Zhang et al [PDF]
  • The Journal of Pathology. 2015 BDNF repairs podocyte damage by microRNA… Min Li et al [PDF]
  • Hypertension. 2015. Neurokinin 3 receptor and phosphocholine transferase… Parchim et al [PDF]
  • Gastroenterology. 2011 Differential T1 interferon-mediated autophagic trafficking… Desai et al [PDF]
  • PLoS Pathog. 2014 Exosomes from hepatitis C infected patients transmit HCV… Bukong et al [PDF]
  • Molecular Therapy Nucleic Acids. 2017. Reverse expression of aging-Associated… Kim H et al [PDF]
  • Diabetologia. 2015 Silencing of miR-195 reduces diabetic… Zheng et al [PDF]
  • Virus Res. 2016 Reversion to wildtype of a mutated… Smithee et al [PDF]
  • RNA. 2010 RNase L releases a small RNA from HCV RNA that refolds… Malathi et al [PDF]
  • Hypertension 2015 Hypoxia-independent upregulation hypoxia f-1 gene expression… Iriyama et al [PDF]

View complete list of citations and references in our Transfection Blog

Visit Altogen’s Transfection Resource: methods, protocols, stable transfection, RNAi … Read more >

Altogen Contract Research Laboratory Services: Altogen Labs provides GLP-compliant laboratory contract research services. Our biology CRO services include xenograft efficacy studies, pharm/tox and safety tests for IND applications, assay development (ELISA, IC50, qPCR), 90+ xenograft animal models, RNAi and gene silencing services. Altogen’s cell culture scientists transform cell lines of choice to stably express a gene of interest via development of a stable cell line in just 28 days.