Description
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Product Availability: In Stock.
Non-silencing control siRNA (5 nmol)
- Non-silencing transfection control siRNA
- Scrambled (non-targeted) sequence with no off-target effects
- MIcrobube includes 5 nmol of ready-to-use chemically synthesized siRNA
- In vitro validated scrambled sequence siRNA
- Not targeting any known human or mouse genes
- Transfection control for chemical transfection and siRNA electroporation applications
- Developed and manufactured by Altogen Biosystems
siRNA Transfection:
Scrambled-non-silencing siRNA: RNAi is a biological phenomenon where double-stranded RNA (dsRNA) specifically reduces the expression of its corresponding gene. Effective inhibition of specific gene expression is experimentally achieved by the transfection of small interfering RNA (siRNA), which binds to the RISC complex, leading to the degradation of target complementary mRNA molecules in the cell. However, when using scrambled-non-silencing siRNA, which serves as a control, the siRNA sequence is designed not to target any specific mRNA, thus not reducing gene expression. This type of siRNA is crucial for validating RNAi experiments by demonstrating that the observed effects are due to specific gene silencing and not off-target effects or cytotoxicity. The success of RNAi experiments depends on the efficient delivery of siRNA into cells via transfection of stable and functional siRNA molecules. Most DNA transfection reagents are unsuitable for siRNA, as they are sensitive to serum or can induce cytotoxicity, negatively impacting gene expression studies. Specialized transfection reagents have been developed and optimized for siRNA transfection, allowing efficient transfection even in the presence of serum, without compromising transfection efficiency or cell viability. Ready-to-use transfection protocols eliminate the need for extensive siRNA optimization experiments.
Selected siRNA citation references:
- Cell Death Differ. 2016. 23(5):787-800. Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells. Dong et al [PDF]
- British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]
- Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 5 nmol (Catalog #4063)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.