Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Apoptosis Inducing siRNA (5 nmol)
Product Description:
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siRNA used as a transfection control or apoptosis-inducing siRNA control
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Microtube includes 5 nmol of ready-to-use chemically synthesized siRNA
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siRNA induce apoptosis when transfected in vitro
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Validated for use in human and mouse cell lines
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Transfection control for chemical transfection and siRNA electroporation applications
- Developed and manufactured by Altogen Biosystems
siRNA Transfection of Apoptosis-inducing siRNA:
Apoptosis-inducing siRNA: Gene silencing by RNA Interference (RNAi) is a powerful technique for studying gene function in mammalian cells, particularly in the context of inducing apoptosis. RNAi is a biological process where double-stranded RNA (dsRNA) specifically reduces gene expression of its corresponding gene. Effective inhibition of specific gene expression is achieved by the transfection of small interfering RNA (siRNA), which binds to the RISC complex, leading to the degradation of target complementary mRNA molecules in the cell. The success of RNAi experiments, especially for inducing apoptosis, depends on the efficient delivery of siRNA into cells via transfection of stable and functional siRNA molecules. Most DNA transfection reagents are unsuitable for siRNA, as they are sensitive to serum or can induce cytotoxicity, negatively impacting gene expression studies. Specialized transfection reagents have been developed and optimized for siRNA transfection, allowing efficient transfection even in the presence of serum, without compromising transfection efficiency or cell viability. Ready-to-use transfection protocols eliminate the need for extensive siRNA optimization experiments. Altogen Biosystems kits are also compatible with the transfection of small RNA (sh/miRNA) molecules, mRNA and plasmid DNA.
Altogen Biosystems siRNA article citation references:
- Cell Death Differ. 2016. 23(5):787-800. Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells. Dong et al [PDF]
Altogen Biosystems manufacturers over 100 pre-optimized in vitro transfection kits for cancer cell lines and primary cells, elecroporation products, and tissue-targeted in vivo delivery reagents for life science research. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 5 nmol (Catalog #4062)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.