Description
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DNA Electroporation Buffer (30 ml)
- Certified RNAse-, DNAse-free electroporation buffer
- Optimized for efficient DNA transfer
- Buffer formulation mimics cellular cytoplasm composition enhancing electroporation pore resealing
- Validated for use with human cell lines
- High viability and cell recovery rates
- Developed and manufactured by Altogen Biosystems
DNA Electroporation Buffer
The DNA electroporation buffer is a specialized buffer used to prepare cells for electroporation with DNA molecules. The buffer is designed to maintain the integrity of the cell membrane during the electroporation process, while also facilitating the uptake of the DNA molecule of interest.
The composition of the DNA electroporation buffer may vary depending on the cell type and the DNA being delivered, but typically includes the following components:
- Low salt concentration: A low salt concentration is used to minimize the osmotic shock to the cells during electroporation.
- Neutral pH: The buffer is adjusted to a neutral pH to maintain the stability of the DNA molecule of interest and prevent damage to the cells.
- Supplemental agents: Additional agents, such as glycerol or sucrose, may be added to the buffer to protect the cells and the DNA from the effects of electroporation.
- Antibiotics: Antibiotics may be added to the buffer to prevent contamination of the cells with bacteria or fungi.
It is important to optimize the composition and concentration of the DNA electroporation buffer to ensure efficient delivery of the DNA into the cells while minimizing toxicity or other adverse effects. Careful attention should be given to the preparation of the buffer and the handling of the cells to ensure the success and reproducibility of the experiment.
Altogen Biosystems manufacturers over 100 pre-optimized in vitro transfection kits for cancer cell lines and primary cells, elecroporation products, and tissue-targeted in vivo delivery reagents for life science research. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 30 ml (Catalog #4067)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.