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DNA Gel Loading Dye Recipes
Agarose Gel Loading Dye Recipes (6x)
When considering which DNA loading dye to use it’s important to select a dye that won’t obscure your sample. i.e. If looking for a product expected to be ~300 bp, bromophenol blue will run with your sample and may obscure it. With 6x dye, load equivalent ratio of 5 µL dye to 25 µL sample.
Dye | 0.5-1.5% | 2.0-3.0% |
Xylene cyanol | 10k-4k bp | 750-200 bp |
Cresol red | 2k-1k bp | 200-125 bp |
Bromophenol blue | 500-400 bp | 150-50 bp |
Orange G | <100 bp | |
Tartrazine | <20 bp | <20 bp |
Recipe 1:
- 0.25 g bromophenol blue
- 3 mL glycerol
- 7 mL H2O
Recipe 2:
- 4 g sucrose
- 0.25 g bromophenol blue or xylene cyanol
- QS with H2O to 10 mL
Recipe 3:
- 60% v/v glycerol
- 20 mM Tris-HCL
- 60 mM EDTA
- 0.48% SDS
- 0.03% xylene cyanol
- 0.03% bromophenol blue
- 0.12% Orange G