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Transfection Reagent for SK-OV-3 Cells (Human Osteosarcoma Cells, HTB-77)
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 74% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems SK-OV-3 Transfection Protocol: [PDF]
Download MSDS: [PDF]
SK-OV-3 Cell Line:
The SK-OV-3 cell line was established in 1973 using the ovarian tissue derived from a sample removed from a 64-year-old Caucasian female who suffered from adenocarcinoma (cancer). This tumorigenic cell line exhibits epithelial cell morphology, and can be used in scientific research by acting as a transfection host. SK-OV-3 cells have been known to be resistant to tumor necrosis, as well as several other cytotoxic drugs such as Adriamycin, cis-platinum, and diphtheria toxin.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the SKOV3 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents (peg-liposome, nanoparticle, lipid, and polymer-based; and tissue targeted: liver, pancreas,kidney). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Research Services:
Altogen Labs is a GLP compliant laboratory that provides preclinical contract research services (CRO). Pharma and biotechnologyservices include: transient and stable transfection (development of stable cell lines), liposome encapsulation (proteins, DNA, mRNA, siRNA), A-to-Z RNAi services, in vivo and in vitro toxicology, xenograft animal models, assay development, cell bankingand cryopreservation services.
- 0.5 ml (Catalog #6918)
- 1.5 ml (Catalog #6919)
- 8.0 ml (Catalog #7089)