Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Electroporation Kit for Ramos Cells (Lymphoma Cells, CRL-1596)
• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems
Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette
Electroporation Efficiency:
Electroporation kit provides 70-85% delivery efficiency of siRNA. Electroporation efficiency was determined by RT-PCR.
Electroporation Protocol and SDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download SDS: [PDF]
Ramos Cell Line:
The Ramos (RA 1) cell line was established from a 3-year-old Caucasian male patient who suffered from Burkitt’s lymphoma. The morphology of this cell line is like that of a lymphoblast tumorigenic tissue. These cells have been tested as negative for Epstein-Barr virus. These cells are useful for biomedical research related to cancers of the lymphatic system. Ramos cells are a type of B-cell lymphoma cell line that was derived from a patient with Burkitt’s lymphoma. These cells are commonly used in research studies to study the biology of lymphomas and to test the efficacy of potential therapies. Ramos cells are known to have several genetic abnormalities, including the translocation of the c-Myc gene on chromosome 8 to the immunoglobulin heavy chain (IgH) gene on chromosome 14, which is a hallmark of Burkitt’s lymphoma. These cells also express high levels of surface immunoglobulin and CD19, which are B-cell markers. Ramos cells have been used in a wide range of studies, including those focused on understanding the mechanisms of oncogenic transformation, identifying new therapeutic targets, and testing the efficacy of immunotherapies.
Mutations:
| PGR | 5241 | 37 | 11 | 100922293 | 100922293 | Missense_Mutation | SNP | C | T |
| SMARCA4 | 6597 | 37 | 19 | 11141426 | 11141426 | Missense_Mutation | SNP | C | T |
| CYP4Z1 | 199974 | 37 | 1 | 47548050 | 47548050 | Missense_Mutation | SNP | C | T |
| H2AFY2 | 55506 | 37 | 10 | 71868913 | 71868913 | Silent | SNP | G | A |
| TCERG1L | 256536 | 37 | 10 | 132944830 | 132944830 | Silent | SNP | G | A |
| TP53 | 7157 | 37 | 17 | 7577520 | 7577520 | Missense_Mutation | SNP | A | T |
| ACSL1 | 2180 | 37 | 4 | 185689471 | 185689471 | Splice_Site | SNP | C | T |
| MMEL1 | 79258 | 37 | 1 | 2541231 | 2541231 | Missense_Mutation | SNP | G | A |
| GPR153 | 387509 | 37 | 1 | 6314852 | 6314852 | Silent | SNP | C | T |
| PLOD1 | 5351 | 37 | 1 | 12014910 | 12014910 | Missense_Mutation | SNP | C | A |
Data:
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 4 ml (Catalog #6583)
- 30 ml (Catalog #6584)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.