DU145 Transfection Kit (Prostate Carcinoma Cells)

Description

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Product Availability: In Stock.

Transfection Reagent for DU145 Cells (Prostate Carcinoma Cells, HTB-81)

• Proprietary cationic lipids formulation
• High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
• Effective and robust intracellular delivery
• Work in the presence of serum
• A proven reagent for establishing stable DU145 cell lines
• Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
• Download DU145 transfection protocol: [PDF]
• Download DU145 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for DU145 cells transfection kit: [PPT]
• UPC/GTIN/EAN: 860002089757
• Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 75% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Product Description:

High efficiency transfection kit for DU145 cells, a human prostate cancer cell line. Transfection reagent supplemented by complex condenser and transfection enhancer.

Transfection Protocol and SDS:

Download Altogen Biosystems Transfection Protocol: [PDF]

Download SDS: [PDF]

DU145 Cell Line:

According to American Cancer Society, over 161,000 men are estimated to be diagnosed with prostate cancer by the end of 2017. It is the third primary cause of male cancer deaths, in the United States. Prostate cancer is the most prevalent cancer especially in older males and can be successfully cured if diagnosed early. However, when it has spread to other parts of the body, more complicated treatment is required. The DU-145 prostate cancer cell line comprises androgen independent cells with a moderate ability to metastasize. The DU-145 cell line was established from human prostate cancer cells. These cells exhibit an epithelial cell morphology and possess metastatic potential. DU-145 cells are commonly used, along with PC3 cell line, in the study of prostate cancer, which affects many people (mainly men over the age of 50). This cell line is considered one of the “classical” prostate cancer cell lines. These cells are not sensitive to hormones and fail to express prostate-specific antigen (PSA). DU-145 cells are epithelial-like adherent cells growing as monolayers. The DU-145 line is not measurably hormone sensitive. It is only weakly positive for acid phosphatase, and isolated cells form colonies in soft agar. Analyses of the cell line have revealed microvilli, tonofilaments, and desmosomes. DU-145 do not express prostate antigen but do express functional receptor activator of NF-kappaB and androgen receptor. It has been shown glucosamine suppresses proliferation of DU-145. Transfection studies carried out using established cell lines significantly aid cancer research. Altogen Biosystems offers efficient lipid-based transfection reagent kits for the DU-145 prostate cancer cell line.

DU145 is a human prostate cancer cell line that was derived from a metastasis tissue of a 69-year-old Caucasian male patient. The DU145 cell line is widely used as a model system for studying prostate cancer, as it exhibits many of the characteristics of aggressive, androgen-independent prostate cancer. DU145 cells have been used in a variety of studies to investigate the molecular and genetic mechanisms underlying prostate cancer development and progression. They have also been used to evaluate the efficacy of potential therapeutic agents for prostate cancer.

Mutations:

Data:

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into DU-145 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

Figure 2. Protein expression of Lamin A/C in DU145 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into DU145 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):

• Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]
• Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
• Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
• J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]
• Mol Cell Biol. 2013 33(7). SCO2 induces p53-mediated apoptosis by Thr845 phosphorylation … Madan et al [PDF]
• Hypertension. 2015 65(2):430-9. Neurokinin 3 receptor and phosphocholine transferase… Parchim et al [PDF]

Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #1749)
• 1.5 ml (Catalog #1750)
• 1.5 ml CRISPR (Catalog #2140)
• 8.0 ml (Catalog #7038)

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.