Category Archives: Uncategorized

Citations and References: Altogen Transfection Products

Citations and References: Altogen® Transfection Reagents

J Cereb Blood Flow Metab 2017. Inhibition of Src family kinases improves cognitive function after intraventricular … Liu et al [PDF]

Nature Medicine 2016. The long noncoding RNA Chaer defines an epigenetic checkpoint in cardiac hypertrophy. Wang et al [PDF]

Molecular Therapy Nucleic Acids 2017. Reverse Expression of Aging-Associated Molecules through Transfection … Kim et al [PDF]

The Journal of Pathology 2015 235(5):731–744. BDNF repairs podocyte damage by microRNA-mediated … Min Li et al [PDF]

Nature Communications 2016. miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy. Badal et al [PDF]

Journal of the American Society of Nephrology 2016. Synaptopodin Limits TRPC6 Podocyte Surface Expression … Yu et al [PDF]

Cardiovascular Research. 2016. Modulators of ventricular apoptosis in a rat model of … Zungu-Edmondson et al [PDF]

Hypertension 2015. Hypoxia-independent upregulation of placental hypoxia factor-1 gene expression … Iriyama T et al [PDF]

Diabetologia 2015. Silencing of miR-195 reduces diabetic cardiomyopathy in C57BL/6 mice. Zheng et al [PDF]

J Virol 2015; 89(23):11761-72. Mutational Disruption of cis-Acting Replication Element 2C in Smithee S et al [PDF]

J Biol Chem 2017 292(2):407-416. Protein Phosphotyrosine Phosphatase 1B in Calpain-dependent … Zhang et al [PDF]

Am J Pathol 2016. Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for … Tomasi et al [PDF]

Virus Res 2016 Jul 15;220:136-49. Reversion to wildtype of a mutated and nonfunctional … Smithee et al [PDF]

Nature 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]

PLoS ONE 2011. Distinctive Patterns of USP19-Mediated Growth Regulation in Normal and Malignant Cells … Lu et al [PDF]

FEBS J 2016. Transforming growth factor B1 antagonizes the transcription expression and vascular signaling … Sen et al [PDF]

Journal of Experimental Medicine 2015. The Dishevelled-binding protein CXXC5 negatively regulates … Kim et al [PDF]

Am J Pathology 2011. Defective myofibroblast formation from mesenchymal stem cells in the aging murine … Cieslik et al [PDF]

Endocrine-Related Cancer 2016. 23(8):595-607. Role of protein S in castration-resistant prostate cancer-like cells. Ning et al [PDF]

PLoS One 2014 SOX2 expression regulated by BRAF and contributes to poor prognosis in colorectal cancer. Lundberg et al [PDF]

BMC Cancer. (2017) 17: 169. Altering calcium influx for selective destruction of breast tumor. Yu et al [PDF]

Rep Biochem Mol Biol. 2015 3(2):76-81.Expression of TGF-β3 in isolated fibroblasts from foreskin. Mahmoudi et al [PDF]

J S Med. 2016 Dec;13(12):1834-1843. Off-Target Effect of Sildenafil on Postsurgical … Salmasi et al [PDF]

International Journal of Pharmaceutical Sciences. 2012. (3):4. Therapeutic Applications of siRNA: A Review. Kumar N et al [PDF]

Nature Methods. 2009. 6:305-309. Journeys across the membrane. Nathan Blow [PDF]

Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]

Hypertension. 2014 63(2):353-61. Tissue transglutaminase contributes to … Liu et al [PDF]

PLoS Pathogens. 2012 8(8) Uridine composition of the poly-U/UC tract of HCV RNA … Schnell et al [PDF]

J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]

J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]

Hypertension. 2015 65(2):430-9. Neurokinin 3 receptor and phosphocholine transferase… Parchim et al [PDF]

PLoS Pathog. 2014 10(10) Exosomes from hepatitis C infected patients transmit HCV … Bukong et al [PDF]

Journal of General Virology. 2008 89:1978. Role of retinoic acid inducible gene-I in human … Liao et al [PDF]

PLoS One. 2012 (7): Pharmacological Inhibition of Microsomal Prostaglandin E Synthase-1 … Finetti et al [PDF]

Journal of Neuroscience. 2013 33(44). Foxo3a Transcriptionally Upregulates AQP4 and Induces … Kapoor et al [PDF]

PLOS ONE. 2014. SOX2 Expression Is Regulated by BRAF and … Lundberg et al [PDF]

Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]

Gastroenterology. 2011 141(2) Differential type I interferon-mediated autophagic trafficking … Desai et al [PDF]

The American Journal of Pathology. 2011 179(4). Defective Myofibroblast Formation from Mesenchymal Stem Cells … Cieslik et al [PDF]

Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]

Patent WO2013055954A1. Biomarker for abdominal aortic aneurysm. Hua Cai [PDF]

Journal of Biological Chemistry. 2011. Regulatory Factor X1-induced Down-regulation of Transforming … Feng et al [PDF]

RNA. 2010 16(11):2108-19. RNase L releases a small RNA from HCV RNA that refolds … Malathi et al [PDF]

Journal of Molecular Endocrinology 2010. Identification of protein kinases that control ovarian hormone release … Sirotkin et al [PDF]

FEBS Letters 2009 (583). MicroRNA 125a and its regulation of the p53 tumor suppressor gene … Zhang et al [PDF]

Cellular Physiology 2011. Identification of MicroRNAs Controlling Human Ovarian Cell Proliferation … Sirotkin et al [PDF]

Patent WO2010004003A2. Modified peptides as potent inhibitors of the psd-95/nmda receptor interaction. Anders Bach et al [PDF]

Clinical Cancer Research. 2010 16(16). Inhibition of Hypoxia Inducible Factor-1α by Dihydroxyphenylethanol … Terzuoli et al [PDF]

Oncogene. 2012 (31). EGFR signaling upregulates expression of microsomal prostaglandin E … Donnini et al [PDF]

PLoS One. 2011 6(1) Identification of distinctive patterns of USP19-mediated growth regulation in normal and malignant cells … Lu et al [PDF]

Carcinogenesis. 2014. A mechanistic study of the proapoptotic effect of tolfenamic acid … Jeong et al [PDF]

Diabetologia. 2012 55(7):2069-79. The p47phox- and NADPH oxidase organiser 1 … Youn et al [PDF]

Leukemia. 2011 (25). Hypoxia-microRNA-16 downregulation induces VEGF expression … Dejean et al [PDF]

Nature Cell Biology. 2013. Protein interaction switches coordinate Raf-1 and MST2/Hippo … Romano et al [PDF]

BMC Cancer. 2012 12(69). Regulation of p14ARF expression by miR-24 … Kwong-Him To et al [PDF]

Patent WO2011150299A2. Treatment of myocardial infarction and vascular injury with netrin-1. Hua Cai [PDF]

British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]

Data Brief. 2016. Data supporting attempted caveolae-mediated phagocytosis of surface-fixed micro-pillars by human osteoblasts. Moerke et al [PDF]

Clin Lung Cancer. 2015 Nov;16(6):e121-9. miR Profiling Identifies Cyclin-Dependent Kinase 6 Downregulation as a Potential Mechanism of Acquired Cisplatin Resistance in Non-Small-Cell Lung Carcinoma. Bar et al [PDF]

Clin Cancer Res. 2010 Aug 15;16(16):4207-16. Inhibition of hypoxia inducible factor-1alpha by dihydroxyphenylethanol, a product from olive oil, blocks microsomal prostaglandin-E synthase-1/vascular endothelial growth factor expression and reduces tumor angiogenesis. Terzuoli et al [PDF]

Nat Commun. 2016 Jul 6;7:12057. Signal transduction controls heterogeneous NF-κB dynamics and target gene expression through cytokine-specific refractory states. Adamson A et al [PDF]

Cell Death Differ. 2016. 23(5):787-800. Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells. Dong et al [PDF]

Carcinogenesis. 2013. 34; 10:2350–2360. A mechanistic study of the proapoptotic effect of tolfenamic acid: involvement of NF-κB activation. Jeong et al [PDF]

J Mol Cell Cardiol. 2015 Jan; 0: 174–185. Netrin-1 Abrogates Ischemia Reperfusion-induced Cardiac Mitochondrial Dysfunction via Nitric Oxide-dependent Attenuation of NOX4 Activation and Recoupling of NOS. Siu et al [PDF]

Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]

Oxidative Medicine and Cellular Longevity. 2015, 792846. Role of Circulating miRNAs as Biomarkers in … Sarrion et al [PDF]

Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]

Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
Mol Ther Nucleic Acids. 2017; 7:42–52. Targeted Multifunctional Lipid ECO Plasmid DNA Nanoparticles as Efficient Non-viral Gene Therapy … Sun et al [PDF]

Mol Cell Biol. 2013 33(7). SCO2 induces p53-mediated apoptosis by Thr845 phosphorylation … Madan et al [PDF]

AltoFect Transfection Reagent for Primary Cells and Hard-to-transfect Cell Lines

Altogen Biosystems developed AltoFect™ Transfection Reagent – a new generation transfection reagent optimized for high transfection efficiency of primary cells and difficult-to-transfect cell lines. It has been successfully tested to deliver all types of nucleic acids (plasmid DNA constructs, si/miRNA, shRNA, dsRNA, mRNA, etc), small proteins, and negatively charged small molecules. Cancer cell lines and primary cell types, a… Continue Reading

CRISPR/Cas9 Transfection Optimization

Efficient delivery of the CRISPR/Cas9 protein complex can be achieved efficiently through the use of cationic lipid-based transfection reagents. Cells may undergo electroporation to increase the probability of successful transfection. Once inside a target cell, the CRISPR/Cas9 protein complex requires no additional stimulation to start modifying the cell’s genome. Genome modification will occur within one… Continue Reading

Where to purchase cancer cell lines?

Cancer cell lines must be purchased from trustworthy vendors (i.e. ATCC, Sigma-Aldrich, DSMZ), along with signing licenses and agreements.  These large cell bank vendors have established good quality control processes, document controls and facilities that are able to offer well characterized cell lines. Continue Reading

Caspase-3/7 cell line screen for test compound

Caspase 3/7 activity is a cell based assay that measures apoptosis.  Commercially available reagents enable high-throughput screening of apoptosis inducing test compounds or contract research organizations can readily perform the compound screening.  A brief overview of the protocol is as follows: Cells in logarithmic growth are seeded in a 96-well plate Depending on the scope… Continue Reading

Tissue culture using flasks vs dishes for cell lines

Flasks and dishes that are cell culture coated are both acceptable consumables to use for cell culture.  Employing sterile techniques allows an experienced scientist to use either item, with pros and cons listed here for each system. Filtered Flasks Narrow opening translates to a lesser chance of contaminant entering the flask Ideal for long term… Continue Reading

Cell passage: How to correctly dilute and split cultured cells

There are many different cell culture techniques. Utilizing correct cell passaging methods is important to keep cell line in exponential growth curve, therefore making it a good model as a host for transfection experiments. “Cell passage” is a term used by other scientists to demonstrate the following process: Wash cells with PBS Detach cells from… Continue Reading

How to ship cells as a live culture?

Shipping cells to collaborators can be accomplished by either sending them a frozen aliquot on dry ice or by sending a live culture.  Sending a live culture has the advantages of the cells continuing their exponential growth to aid in timing of experiments.  This is accomplished by completely filling the flask with culture medium, closing… Continue Reading

How to determine cell viability?

Cell viability is a calculation of the number of viable or living cells within the total number of cells.  Although there are now commercially available alternatives to the historical method, trypan blue exclusion is the tried and true method commonly used in the lab.  Briefly, trypan blue dye is added to a cell suspension and… Continue Reading

How to determine mycoplasma contamination?

Mycoplasma contamination is a common occurrence and labs must be vigilant in monitoring and combating contamination issues.  Mycoplasma are bacteria extremely difficult to detect in cultured cells by visual inspection.  Many labs do not incorporate recurring mycoplasma testing until the lab experiences a contamination incident, at which time cleaning and testing procedures are put in… Continue Reading

Even cell distribution in the 6-well plate instead of a ring of cells around the edges?

Performing cell culture experiments in round well plates can often lead to troubling spatial dispersion of the cells.  This can be attributed to culture incubators having high vibrations, non-level incubators or bad practices such as swirling the plate after adding cells.  In order to achieve even distribution of cells in the wells of culture plates,… Continue Reading

Is it necessary to synchronize cells?

Exponentially growing cultured cells are growing at difference cell cycle stages, termed asynchronous.  Scientists are vigilant in their techniques to ensure they are using non-confluent cells prior to their experiments; thus, synchronization is not commonly employed.  However, synchronizing your cells can help improve the results of certain cell based assays such as viral integration or… Continue Reading

Low cell viability due to the freezing or thawing of cancer cell lines

Freeze slow, thaw fast.  The process of freezing and thawing cancer cell lines requires extreme attention to protocol.  For freezing, a good freezing media that contains DMSO is added to the correct number of cells following the manufacturer’s instructions.  The vital part of the freezing process is to ensur that the freezing process is slow. … Continue Reading