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Transfection Reagent for BT-20 Cells (Breast Carcinoma Cells, HTB-19)
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Download PowerPoint presentation for BT-20 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems Transfection Protocol: [PDF]
Download MSDS: [PDF]
BT-20 Cell Line:
Breast cancer represents the most prevalent type of cancer among women worldwide, making it a significant public health concern. Despite major improvements in breast cancer management, preclinical breast cancer research plays an essential role in the development of novel treatments. Triple Negative Breast Cancer (TNBC) cell lines are one of the most valuable tools of research during preclinical studies. Unlike, standard breast cancer cells, TNBC cells lack the expression of estrogen and progesterone receptors. Also, these cells do not exhibit amplification of HER2, a type of Epidermal Growth Factor Receptors (EGFR), which makes TNBC cells non-receptive to many forms of treatment that hormone-positive cells respond well. However, it makes TNBC cell lines a powerful research tool for new types of gene therapy. The BT-20 cell line was established from a primary human breast cancer tumor in 1958 by isolation and culturing of cells and has proven to be a successful host for a variety of transfection studies. These cells came from a 74-year-old Caucasian female patient with breast carcinoma and were obtained by E.Y. Lasfargues and L. Ozzello. Growth factors are often used when studying these cells that do not possess estrogen receptors but do express an estrogen receptor mRNA that has a deletion of exon 5. Altogen Biosystems provides two-component lipid-based transfection reagent kits for the BT-20 breast cancer cell line, which have shown high transfection efficacy verified by qRT-PCR. The BT-20 transfection kit is particularly helpful in the field of biomedical therapeutics as well as in gene expression studies.
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into BT20 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in BT-20 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into BT-20 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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