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Transfection Reagent for CT26.WT Cells (Colon Carcinoma Cells, CRL-2638)
Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Download PowerPoint presentation for CT26WT cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 92% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems CT26WT Transfection Protocol: [PDF]
Download MSDS: [PDF]
CT26.WT Cell Line:
The CT26.WT cell line is a colon carcinoma cell line, which was cloned with the retroviral vector LXSN, which contains the lacZ gene. This cell line exhibits fibroblast morphology, and is tumorigenic. This cell line could be useful in research related to medical treatments for colon carcinoma (cancer), which affects many people every year. CT26.WT is often used as a model for the testing of immunotherapy protocols, as well as transfection host cell line for research studies on immune response.
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in CT26WT cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).
Figure 2. Protein expression of Cyclophilin B in CT26.WT cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into CT26.WT cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, electroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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