HCT-116 Transfection Kit (Colon Carcinoma)

Description

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.

Transfection Reagent for HCT116 Cells (Colon Carcinoma Cells, CCL-247)

• Proprietary cationic lipids formulation
• High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
• Effective and robust intracellular delivery
• Kit includes Transfection Enhancer reagent
• Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• A proven reagent for establishing HCT116 stable cell lines
• Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
• Download in vitro HCT116 transfection protocol: [PDF]
• Download HCT-116 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for HCT-116 cells transfection kit: [PPT]
• UPC/GTIN/EAN: 860002089762
• Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Product Description:

Transfection kit is specially designed for the HCT116 cell line, a human colorectal carcinoma cells. Transfection reagent ensures high efficiency in delivering both DNA and RNA into these cells.

Transfection Protocol and SDS:

Download Altogen Biosystems HCT116 Transfection Protocol: [PDF]

Download SDS: [PDF]

HCT-116 Cell Line:

Colon cancer mostly affects older people in their late 60s and early 70s; however, the only group that is associated with increased incidence rates is people younger than age 50, as per the Colon Cancer Alliance (CCA). The HCT-116 colon cancer cell line is essential for researching biochemical pathways that aid the proliferation of colon carcinoma. The HCT-116 cell line was derived from epithelial tumorigenic colon tissue of an adult male with colorectal carcinoma. These cells have a mutation in the 13 codon of the ras proto-oncogene, and therefore they are useful for PCR assays studying this codon. They are also positive for transforming growth factor beta 1 and beta 2 expression. HCT-116 cells are known for their sensitivity to chemotherapeutic drugs such as 5-fluorouracil and oxaliplatin, which makes them a valuable tool for evaluating the efficacy of new cancer treatments. This cell line is useful for biomedical research related to colon cancer. Altogen Biosystems provides highly efficient lipid-based transfection reagent kits for the HCT-116 colon cancer cell line.

HCT116 is a human colorectal carcinoma cell line commonly used in research studies. The HCT116 cell line was derived from the colon of a 44-year-old male in 1982. It has been extensively characterized and is known for its ability to form tumors in vivo, making it a useful tool for studying cancer biology and for testing anti-cancer drugs. The HCT116 cell line has a diploid karyotype and is deficient in the mismatch repair gene MLH1, which makes it a useful model for studying the genetic basis of colorectal cancer. The cells are adherent and grow well in culture, and can be maintained in a variety of media and conditions. HCT116 cells have been used in numerous studies investigating cancer biology, including DNA repair mechanisms, cell cycle regulation, apoptosis, and drug resistance.

Mutations:

Data:

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into HCT-116 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

Figure 2. Protein expression of Lamin A/C in HCT-116 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into HCT-116 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #6811)
• 1.5 ml (Catalog #6812)
• 1.5 ml CRISPR (Catalog #2148)
• 8.0 ml (Catalog #7045)

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.