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Transfection Reagent for Hepa 1-6 Cells (Hepatoma Cells, CRL-1830)
- Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Kit includes Transfection Enhancer reagent
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
Download PowerPoint presentation for Hepa cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 75% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems Hepa Transfection Protocol: [PDF]
Download MSDS: [PDF]
Hepa 1-6 Cell Line:
The Hepa 1-6 cell line is derived from the BW7756 mouse (Mus musculus) hepatoma that arose from a C57/L mouse. It was originally established from mouse liver cells, which suffered from hepatoma (liver cancer). The cells are tumorigenic and exhibit epithelial morphology. Hepa 1-6 cells make a good transfection host, and have been tested negative for mousepox. Hepa 1-6 cell line used in biomedical research to study mammalian liver tissue functions.
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into Hepa cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A/C in Hepa 1-6 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into Hepa 1-6 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and pre-optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits and reagents. Advanced formulation of reagents and optimized transfection protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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