Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Electroporation Kit for HL-60 Cells (Plasmacytoma Cells, CCL-240)
• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems
Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette
Electroporation Efficiency:
Electroporation kit provides 65-80% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.
Electroporation Protocol and SDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download SDS: [PDF]
HL-60 Cell Line:
The tumorigenic HL-60 cell line was established using the peripheral blood tissue from a 36-year-old female patient who suffered from acute promyelocytic leukemia (APL). This cell line exhibits myeloblastic cell morphology and a promyeloblast cell type. These cells positively express the myc oncogene, and was derived by S.J. Collins, et all. HL-60 have been known to spontaneously differentiate, this can even be stimulated and controlled, to an extent, by a variety of compounds. This cell line is useful as a transfection host in scientific research. HL60 cells are known for their ability to differentiate into various types of blood cells, which makes them a valuable tool for studying hematopoiesis and blood cell development. Researchers use HL60 cells to investigate the molecular mechanisms underlying leukemia development and progression, and to identify potential therapeutic targets and biomarkers of the disease.
Mutations:
| GRID1 | 2894 | 37 | 10 | 87484145 | 87484145 | Missense_Mutation | SNP | C | T |
| KLK6 | 5653 | 37 | 19 | 51470537 | 51470537 | Missense_Mutation | SNP | C | T |
| SEMG2 | 6407 | 37 | 20 | 43851147 | 43851147 | Missense_Mutation | SNP | C | T |
| PRDM16 | 63976 | 37 | 1 | 3102712 | 3102712 | Missense_Mutation | SNP | A | G |
| FUCA1 | 2517 | 37 | 1 | 24175161 | 24175161 | Missense_Mutation | SNP | C | G |
| AHDC1 | 27245 | 37 | 1 | 27878412 | 27878412 | Missense_Mutation | SNP | C | T |
| TSSK3 | 81629 | 37 | 1 | 32829236 | 32829236 | Silent | SNP | C | T |
| CPT2 | 1376 | 37 | 1 | 53679242 | 53679242 | Missense_Mutation | SNP | T | C |
| CYP2J2 | 1573 | 37 | 1 | 60366662 | 60366662 | Missense_Mutation | SNP | C | A |
| CELSR2 | 1952 | 37 | 1 | 109793998 | 109793998 | Missense_Mutation | SNP | G | A |
Data:
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
- 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
- Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]
- Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]
- Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 4 ml (Catalog #1751)
- 30 ml (Catalog #1752)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.