Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Electroporation Kit for Jurkat Cells (T-Cell Leukemia Cells, TIB-152)
• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems
Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette
Electroporation Efficiency:
Electroporation kit provides 75-90% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.
Electroporation Protocol and SDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download SDS: [PDF]
Jurkat Cell Line:
The Jurkat cell line was established in 1970 from the peripheral blood of a patient with acute T cell leukemia. Jurkat cells used in cell biology research to study cancer of blood cells (leukemia). Jurkat cells provide a model to study T cell signaling, expression of chemokine receptors susceptible to viral entry, and the effects of radiation and drugs. Jurkat cell line is also known to produce interleukin 2. Derivatives of Jurkat cell line include JCaM1.6, J.RT3-T3.5, D1.1, J.gamma-1, I 9.2 and I 2.1 cell lines.
Jurkat cells are a type of human T lymphocyte cell line that was originally isolated from the peripheral blood of a 14-year-old boy with T cell leukemia. These cells are commonly used in biomedical research to study T cell activation, signal transduction, gene regulation, and various other aspects of cellular biology. Jurkat cells are widely used because they are easy to culture, grow quickly, and can be induced to undergo T cell activation and proliferation in response to a variety of stimuli, such as mitogens, cytokines, and antigens. They have also been extensively characterized at the molecular and cellular levels, making them a valuable tool for understanding the mechanisms of T cell activation and function.
Mutations:
| TP53 | 7157 | 37 | 17 | 7578263 | 7578263 | Nonsense_Mutation | SNP | G | A |
| FBXW7 | 55294 | 37 | 4 | 153247289 | 153247289 | Missense_Mutation | SNP | G | A |
| TP53 | 7157 | 37 | 17 | 7579312 | 7579312 | Splice_Site | SNP | C | T |
| ZC3H4 | 23211 | 37 | 19 | 47572412 | 47572412 | Missense_Mutation | SNP | C | G |
| SMARCA4 | 6597 | 37 | 19 | 11143994 | 11143994 | Missense_Mutation | SNP | G | A |
| ENTPD7 | 57089 | 37 | 10 | 101455842 | 101455842 | Missense_Mutation | SNP | G | A |
| RAB40C | 57799 | 37 | 16 | 677527 | 677527 | Missense_Mutation | SNP | G | A |
| DAZAP1 | 26528 | 37 | 19 | 1421251 | 1421251 | Silent | SNP | C | T |
| MAN1C1 | 57134 | 37 | 1 | 26104628 | 26104628 | Silent | SNP | C | T |
| APC | 324 | 37 | 5 | 112154771 | 112154771 | Nonsense_Mutation | SNP | C | T |
| PTGR1 | 22949 | 37 | 9 | 114348376 | 114348376 | Silent | SNP | C | T |
| PDCL | 5082 | 37 | 9 | 125585446 | 125585446 | Missense_Mutation | SNP | C | T |
| ATN1 | 1822 | 37 | 12 | 7047777 | 7047777 | Missense_Mutation | SNP | G | A |
Data:
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
- 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
- Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]
- Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]
Altogen Biosystems manufacturers over 100 pre-optimized in vitro transfection kits for cancer cell lines and primary cells, elecroporation products, and tissue-targeted in vivo delivery reagents for life science research. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 4 ml (Catalog #6567)
- 30 ml (Catalog #6568)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.