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Transfection Reagent for KB Cells (Mouth Epidermal Carcinoma Cells)
- Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Kit includes Transfection Enhancer reagent
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Work in the presence of serum
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Download PowerPoint presentation for KB cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 73% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems KB Transfection Protocol: [PDF]
Download MSDS: [PDF]
KB Cell Line:
The KB cell line was first believed to be established from a removal of human mouth epidermal carcinoma cells. These cells were derived from human carcinoma of the nasopharynx, a type of throat cancer. KB cells exhibit an epithelial cell morphology, and are known to produce keratin. Many medical applications exist for KB cell line. Recent testing has identified HeLa cell line marker chromosomes in the KB cell line, and through DNA fingerprinting, KB cells are now thought to have been produced due to HeLa cell contamination. Now KB is known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences and have been found to be positive for keratin by immunoperoxidase staining. The KB line is also used as an assay for antineoplastic agents. An endoribonuclease which cleaves tRNA precursor molecules has been partially purified from human KB tissue culture cells. KB cells have also been used by the National Cancer Institute as an antitumor assay for screening plant extracts.
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the KB cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).
Figure 2. Protein expression of GAPDH in KB cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into KB cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems manufacturers preoptimized transfection kits for cancer research. Reagents and transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery into liver, pancreas, and kidney tissues), and in vivo biodistribution reagents (PEG-Liposome, Nanoparticle, Lipid, and Polymer-based kits). Optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, and RNA molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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