Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Electroporation Kit for KG-1 Cells (Myelogenous Leukemia Cells, CCL-246)
• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems
Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette
Electroporation Efficiency:
Electroporation kit provides 70-85% delivery efficiency of siRNA. Electroporation efficiency was determined by qRT-PCR.
Electroporation Protocol and SDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download SDS: [PDF]
KG-1 Cell Line:
The KG-1 cell line was established from the bone marrow of a 59-year-old Caucasian male patient who suffered from acute myelogenous leukemia (cancer of the blood or bone marrow). These cells exhibit myeloblast cell morphology, and express the HLA A30, A31, B35, and Cw4 antigens. KG-1 cells are EBNA negative, and respond well to colony stimulating factor (CSF). These cells are used for cancer biomedical research, especially related to bone marrow and leukemia. KG1 cells are a human myeloid leukemia cell line that was derived from the bone marrow of a patient with acute myeloid leukemia (AML). These cells have been widely used in research as a model system for studying various aspects of leukemia biology, including the molecular mechanisms underlying leukemogenesis and drug resistance. KG1 cells have several properties that make them useful for research. Firstly, they are relatively easy to culture in the laboratory and can be grown in large quantities. Secondly, they have a relatively simple karyotype, which makes them useful for studying chromosomal abnormalities in leukemia. Thirdly, they express high levels of various surface markers that are characteristic of myeloid leukemia, such as CD34 and CD38. Research using KG1 cells has contributed significantly to our understanding of leukemia biology. For example, KG1 cells have been used to study the effects of various chemotherapeutic agents on leukemia cells and to screen potential drug candidates for their efficacy against AML. Additionally, KG1 cells have been used to study the molecular mechanisms underlying leukemia development and progression, including the role of various oncogenes and tumor suppressor genes. KG1 cells have also been used as a model system for studying the effects of various growth factors and cytokines on leukemia cell proliferation and differentiation. For example, studies have shown that treatment with granulocyte-macrophage colony-stimulating factor (GM-CSF) can promote the differentiation of KG1 cells into mature myeloid cells, providing insight into potential therapeutic strategies for AML.
Mutations:
| PTGDR | 5729 | 37 | 14 | 52741530 | 52741530 | Nonsense_Mutation | SNP | C | T |
| FHOD3 | 80206 | 37 | 18 | 34320643 | 34320643 | Missense_Mutation | SNP | G | A |
| DUSP8 | 1850 | 37 | 11 | 1586951 | 1586951 | Missense_Mutation | SNP | C | G |
| GPR153 | 387509 | 37 | 1 | 6314891 | 6314891 | Missense_Mutation | SNP | A | T |
| VPS13D | 55187 | 37 | 1 | 12516153 | 12516153 | Missense_Mutation | SNP | C | T |
| LRRC38 | 126755 | 37 | 1 | 13839711 | 13839711 | Silent | SNP | C | T |
| PRDM2 | 7799 | 37 | 1 | 14107576 | 14107576 | Missense_Mutation | SNP | C | T |
| NCDN | 23154 | 37 | 1 | 36026283 | 36026283 | Silent | SNP | C | T |
| ADPRHL2 | 54936 | 37 | 1 | 36557618 | 36557618 | Silent | SNP | C | T |
| CYP4A22 | 284541 | 37 | 1 | 47607788 | 47607788 | Missense_Mutation | SNP | T | G |
| JUN | 3725 | 37 | 1 | 59248138 | 59248138 | Missense_Mutation | SNP | T | G |
| GBP1 | 2633 | 37 | 1 | 89528786 | 89528786 | Silent | SNP | C | G |
| NOTCH2 | 4853 | 37 | 1 | 120466578 | 120466578 | Missense_Mutation | SNP | T | C |
Data:
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 4 ml (Catalog #6575)
- 30 ml (Catalog #6576)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.