LIPID In Vivo Transfection Reagent

Description

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.

LIPID-based In Vivo Transfection Kit
siRNA and plasmid DNA in vivo delivery reagent

Product Description:

Lipid-based in vivo transfection reagent and enhancer reagent (included in the kit) – an effective delivery system designed for tissue-targeted delivery of cargo biomolecules in mice and rats. Optimized for the effective transfection of small molecules, RNA, DNA, proteins, mRNA, and other negatively charged molecules. Reagent is biodegradable and functionally tested in rodents for high efficacy delivery and minimal immune response. Efficient for delivery into liver, pancreas, kidney, and various tumor types.

Altogen’s LIPID In Vivo Transfection Reagent:

• Cationic lipid liposome-based reagent
• Lipid conjugated complexes are stable in serum for 16h
• Efficient delivery to the liver, pancreas, kidney, and certain tumor types via systemic administration
• Efficient siRNA and plasmid DNA delivery via direct subcutaneous tumor injection (various tumor types)
• Functionally tested in mice
• Applicable for plasmid DNA/siRNA co-injection
• Download LIPID-based in vivo transfection protocol: [PDF] [Word]
• Download PowerPoint presentation for LIPID-based in vivo transfection kit: [PPT]
• Download safety data sheet: [PDF]
• UPC/GTIN/EAN: 860002089700
• Brand: ALTOGEN®, developed and manufactured by Altogen Biosystems

Modes of administration:

• Systemic intravenous (i.v.) injection
• Direct intratumoral (i.t.) injection
• Intraperitoneal (i.p.) injection

DATA

Figure 1. Systemic administration (i.v.) of lipid-based in vivo delivery reagent (cat#5011, Altogen Biosystems) conjugated with siRNA targeting Lamin A/C mRNA, or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Figure 2. Intratumoral administration (i.t.) of lipid-based in vivo transfection reagent (cat#5011, Altogen Biosystems) conjugated with siRNA targeting Lamin A/C mRNA, or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

Figure 3. Systemic administration (i.v.) of Lipid In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA, or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

Selected Lipid Transfection Reagent citations:

• J Biol Chem. 2017 292(2):407-416. Protein Phosphotyrosine Phosphatase 1B in Calpain-dependent … Zhang et al [PDF]
• J Virol. 2015; 89(23):11761-72. Mutational Disruption of cis-Acting Replication Element 2C in Smithee S et al [PDF]
• Am J Pathol. 2016. 186(9): 2326–2336.Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response. Tomasi et al [PDF]
• Virus Res. 2016 Jul 15;220:136-49. Reversion to wildtype of a mutated and nonfunctional coxsackievirus B3CRE(2C). Smithee et al [PDF]
• Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]

All ALTOGEN® IN VIVO Transfection Kits supplied with ready-to-run transfection protocols that eliminate the need for extensive transfection optimization experiments. Read more about transfection technology at Altogen’s Transfection Resource.

Lipid-based in vivo delivery system designed for tissue-targeted delivery of cargo biomolecules for the effective transfection of negatively charged molecules into cells, including DNA and RNA into cells. This reagent is biodegradable and fiunctionally tested in rodents. In the context of tissue engineering, lipid reagent can facilitate the delivery of genes that promote cellular growth and differentiation within specific tissues. The design of lipid reagent ensures hight transfection efficacy of cargo biomolecules into tissues and minimal immune response (tissue toxicity). Reagent provides efficient delivery to the liver, pancreas, kidney, and various tumor types. 

Volume Options:

• 0.5 ml – 10 injections (Catalog #5010)
• 1.5 ml – 30 injections (Catalog #5011)
• 8.0 ml – 160 injections (Catalog #5012)
• 25 ml – 50 rat injections or 500 mouse injections (Catalog #5013)

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.