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Transfection Reagent for PC-12 Cells (Rat Pheochromocytoma Cells, CRL-1721)
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
- Works well for standard reverse transfection and high-throughput applications
- Download PowerPoint presentation for PC-12 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems PC-12 Transfection Protocol: [PDF]
Download MSDS: [PDF]
PC-12 Cell Line:
PC-12 cells are extensively used as a model for neuronal differentiation, investigating the effects of differentiation on exocytotic release, in particular. Not considered adult neurons, PC-12 cells can quickly differentiate into neuron-like cells due to their embryological nature with neuroblastic cells, exhibiting similar to neurons properties. PC-12 was originally derived in 1976 from a transplantable pheochromocytoma of the rat adrenal medulla. PC-12 are small and difficult to transfect cells with a limited amount of cytoplasm. They have an estimated population doubling time of more than two days. PC-12 cells stop dividing and terminally differentiate when treated with nerve growth factor, which makes them useful as a model system for neuronal differentiation. PC-12 treated with nerve growth factor stop proliferation, grow long neuritis, and exhibit cellular composition changes that are associated with neuronal differentiation. When PC-12 differentiate, they are even more difficult to transfect. Both nitric oxide and neuronal nitric oxide synthase are required for the differentiation of PC-12 cells. Research suggests PC-12 cells may represent a stage specific vulnerability to a known neurotoxic agent. The PC-12 cell line has a rounded morphology and tends to form clusters in culture. Also, PC-12 cells are hard to attach to non-coated plastic surfaces, which makes them difficult to culture.
The PC-12 cell line is an invaluable tool for studying adrenal glands as well as malignancies related to them. Also, the PC-12 cell line is a suitable model for studying brain diseases. PC-12 cells are pheochromocytoma cells that are not capable of synthesizing epinephrine. Though they have been known to produce norepinephrine, dopamine, and catecholamines. Cells from the PC-12 rat pheochromocytoma are a mixture of eosinophils and neuroblasts, whose differentiation can be modulated based on research needs. Altogen Biosystems developed lipid-based transfection reagent optimized for the PC-12 cell line.
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into PC-12 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
Figure 2. Protein expression of Lamin A in PC-12 cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into PC-12 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
PC12 Transfection Reagent citation references:
- J S Med. 2016 Dec;13(12):1834-1843. Off-Target Effect of Sildenafil on Postsurgical … Salmasi et al [PDF]
Altogen Biosystems transfection and electroporation products for life sciences and cancer research. Company developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft services (60+ in-house validated xenograft models), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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