Ramos Electroporation Kit (Lymphoma Cells, CRL1596)

$395.00$797.00

Kit Size VolumePricePay with CC, PO, or PaypalAdd to Cart
4 ml (Catalog #6583)$395.00
30 ml (Catalog #6584)$797.00
SKU: RAMOS Category:

Description

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.


Electroporation Buffer

Electroporation Kit for Ramos Cells (Lymphoma Cells, CRL-1596)

• Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
• Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
• Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
• Certified RNAse- and DNAse-free electroporation buffer
• Effective electroporation delivery and high cell viability provides reproducible electroporation results
• High cell viability and superior cell recovery rates
• Electroporation protocols are provided with with the purchase of the Electroporation Kit
• Developed and manufactured by Altogen Biosystems

Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette

Electroporation Efficiency:
Electroporation kit provides 70-85% delivery efficiency of siRNA. Electroporation efficiency was determined by RT-PCR.

Electroporation Protocol and SDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download SDS: [PDF]

Ramos Cell Line:
The Ramos (RA 1) cell line was established from a 3-year-old Caucasian male patient who suffered from Burkitt’s lymphoma. The morphology of this cell line is like that of a lymphoblast tumorigenic tissue. These cells have been tested as negative for Epstein-Barr virus. These cells are useful for biomedical research related to cancers of the lymphatic system. Ramos cells are a type of B-cell lymphoma cell line that was derived from a patient with Burkitt’s lymphoma. These cells are commonly used in research studies to study the biology of lymphomas and to test the efficacy of potential therapies. Ramos cells are known to have several genetic abnormalities, including the translocation of the c-Myc gene on chromosome 8 to the immunoglobulin heavy chain (IgH) gene on chromosome 14, which is a hallmark of Burkitt’s lymphoma. These cells also express high levels of surface immunoglobulin and CD19, which are B-cell markers. Ramos cells have been used in a wide range of studies, including those focused on understanding the mechanisms of oncogenic transformation, identifying new therapeutic targets, and testing the efficacy of immunotherapies.

Mutations:

PGR 5241 37 11 100922293 100922293 Missense_Mutation SNP C T
SMARCA4 6597 37 19 11141426 11141426 Missense_Mutation SNP C T
CYP4Z1 199974 37 1 47548050 47548050 Missense_Mutation SNP C T
H2AFY2 55506 37 10 71868913 71868913 Silent SNP G A
TCERG1L 256536 37 10 132944830 132944830 Silent SNP G A
TP53 7157 37 17 7577520 7577520 Missense_Mutation SNP A T
ACSL1 2180 37 4 185689471 185689471 Splice_Site SNP C T
MMEL1 79258 37 1 2541231 2541231 Missense_Mutation SNP G A
GPR153 387509 37 1 6314852 6314852 Silent SNP C T
PLOD1 5351 37 1 12014910 12014910 Missense_Mutation SNP C A

 

Data:
Ramos Electroporation Kit

Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).

Electroporation Buffer

Volume Options:

  • 4 ml (Catalog #6583)
  • 30 ml (Catalog #6584)

Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.

Product Availability: In Stock.

Additional information

Weight 1 lbs
Dimensions 4 × 3 × 0.2 in
Kit Size (Volume)

4 ml (Catalog #6583), 30 ml (Catalog #6584)