Description
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.
Electroporation Kit for RBL-2H3 Cells (Basophilic Leukemia Cells, CRL-2256)
- Electroporation Kit include: electroporation buffer + buffer-specific optimized electroporation protocols
- Pre-optimized electroporation protocols are provided for delivery of plasmids (DNA), siRNA, mRNA, and proteins
- Proprietary electroporation buffer formulation mimics cellular cytoplasm composition (superior electropore resealing)s
- Certified RNAse- and DNAse-free electroporation buffer
- Effective electroporation delivery and high cell viability provides reproducible electroporation results
- High cell viability and superior cell recovery rates
- Electroporation protocols are provided with with the purchase of the Electroporation Kit
- Developed and manufactured by Altogen Biosystems
Electroporation Buffer:
4 mL of electroporation buffer is sufficient for 20 reactions using standard 0.2 cm elecrtoporation cuvette
30 mL of electroporation buffer is sufficient for 150 reactions using standard 0.2 cm elecrtoporation cuvette
Electroporation Efficiency:
Electroporation kit provides 80-95% delivery efficiency of siRNA. Electroporation efficiency was determined by RT-PCR.
Electroporation Protocol and SDS:
Electroporation protocols are provided with the purchase of the Electroporation Kit
Download SDS: [PDF]
RBL-2H3 Cell Line:
RBL-2H3 was established in 1978 in the Laboratory of Immunology at the National Institute of Dental Research from basophilic leukemia cells in a Wistar Rat (Rattus norvegicus). These cells are useful because they can secrete histamine and other mediators. They have been useful to study of secretion in mast cells. Although nearly all lots of fetal bovine serum support the growth of these cells, the cells grown in some lots degranulate better after FcERI aggregation. RBL-1 (CRL-1378) is another basophilic leukemia cell line that does not degranulate. RBL-2H3 is a cell line that is commonly used in research related to allergies, inflammation, and mast cell biology. These cells were derived from a rat basophilic leukemia (RBL) tumor and are widely used as a model for studying mast cell function and allergic responses. RBL-2H3 cells have several characteristics that make them useful in research. These cells express high levels of IgE receptors (FcεRI) on their surface, which allows them to respond to allergens and trigger mast cell degranulation. RBL-2H3 cells also secrete a range of mediators and cytokines, including histamine, leukotrienes, and interleukins, which are important in the allergic response. Researchers use RBL-2H3 cells to study the mechanisms underlying mast cell activation and degranulation, the role of specific molecules and pathways in allergic responses, and to test potential therapeutics for allergic diseases. Overall, RBL-2H3 cells are an important tool for advancing our understanding of allergy and mast cell biology.
Data:
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
- 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
- Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]
- Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]
- Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, elecroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource. Altogen Labs provides safety and efficacy preclinical research services. GLP-compliant studies for IND applications, and drug development, including over 90 in-house validated xenograft models, safety toxicology, etc (visit AltogenLabs.com).
Volume Options:
- 4 ml (Catalog #6587)
- 30 ml (Catalog #6588)
Purchase Orders: Click “Add to Cart” button to order, then email PO to orders@altogen.com.
Product Availability: In Stock.