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Transfection Reagent for SK-N-MC Cells (Neuroblastoma Cells)
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
- Download PowerPoint presentation for SKNAS cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 76% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems SK-N-MC Transfection Protocol: [PDF]
Download MSDS: [PDF]
SK-N-MC Cell Line:
SK-N-MC was derived in 1971 from a 14-year-old girl. The original paper indicated it came from the supraorbital metastasis of a neuroblastoma. However, SK-N-MC is now thought by many to have originated from an Askin’s tumor related to Ewing’s sarcoma, which is the morphologically similar to neuroblastoma. Research showed FLI1-EWS gene fusion, the molecular pathological change in Ewing’s sarcoma and Askin’s tumor, in the cells. SK-N-MC are adherent, fibroblast-like cells growing as monolayers. The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range. SK-N-MC has a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. It has moderate dopamine-beta-hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines. SK-N-MC cells exhibit an epithelial cell type, and are neuroblastoma cells, which makes them useful for research related to brain cancers.
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the SKNMC cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).
Figure 2. Protein expression of GAPDH in SK-N-MC cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into SK-N-MC cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):
- Nature. 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
- Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]
- Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]
- Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]
- RNA. 2010 16(11):2108-19. RNase L releases a small RNA from HCV RNA that refolds … Malathi et al [PDF]
- Gastroenterology. 2011 141(2) Differential type I interferon-mediated autophagic trafficking … Desai et al [PDF]
- PLoS Pathog. 2014 10(10) Exosomes from hepatitis C infected patients transmit HCV … Bukong et al [PDF]
Altogen Biosystems provides preoptimized transfection products for life science research applications. Transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery to liver, pancreas, and kidney tissues), and broad range in vivo delivery reagents (PEG-Liposome, Nanoparticle-based, Lipid-based, and Polymer-based kits). Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, RNA, and any other negatively charged molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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