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Transfection Reagent for VERO Cells (African Green Monkey Kidney Cells)
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Download PowerPoint presentation for VERO cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems VEROTransfection Protocol: [PDF]
Download MSDS: [PDF]
VERO Cell Line:
The Vero lineage was isolated from kidney epithelial cells extracted from Cercopithecus aethiops (African green monkey). The lineage was developed by Yasumura and Kawakita in 1962. The original cell line was named “Vero” from “Verda Reno” (green kidney in esperanto). Vero cells are used for multiple purposes, including screening for the toxins, as a host cell line for growing virus, as a host cell line for eukaryotic parasites (Trypanosomatids.) The Vero cell lineage is aneuploid and continuous. It can be replicated through numerous cycles of division without becoming senescent. Aneuploidy means having an abnormal number of chromosomes. Unlike normal mammalian cells Vero cells are interferon-deficient. They don’t produce type 1 interferons when infected.
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the VERO cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).
Figure 2. Protein expression of GAPDH in VERO cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into VERO cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, electroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Labs Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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