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Transfection Reagent for Weri-Rb-1 Cells (Retinoblastoma Cells)
- Proprietary cationic lipids formulation
High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
Effective and robust intracellular delivery
Kit includes Transfection Enhancer reagent
Produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Work in the presence of serum
A proven reagent for establishing stable cell lines
Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Download PowerPoint presentation for Weri-Rb-1 cells transfection kit: [PPT]
Developed and manufactured by Altogen Biosystems
Reagent exhibits at least 75% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems WeriRb1 Transfection Protocol: [PDF]
Download MSDS: [PDF]
Weri-Rb-1 Cell Line:
WERI-Rb-1 was derived in 1974 from retinoblastoma tissue of a 1-year-old Caucasian girl in 1974. This is a near diploid line. The modal chromosome number is 47 occurring at 38 percent and the rate of polyploidy is nine percent. Fifteen to sixteen marker chromosomes are present in all cells. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations. The cells survive culturing in Difco Bacto-Agar but do not form colonies. The cells grow in grape-like clusters of round cells in suspension. Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli. The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations.
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into Weri-Rb1 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Figure 2. Protein expression of Lamin A in Weri-Rb-1 cells. DNA plasmid expressing Lamin A or siRNA targeting Lamin A were transfected into Weri-Rb-1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Selected Weri-Rb1 transfection reagent citations:
- BMC Cancer. 2012 12(69). Regulation of p14ARF expression by miR-24 … Kwong-Him To et al [PDF]
- British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]
- Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
Altogen Biosystems transfection and electroporation products for life sciences and cancer research. Transfection reagents are developed for individual cancer cell line and transfection protocols are optimized for maximum delivery efficiency. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged biomolecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.
Altogen Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
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