SDS Gel Dyes & Buffers

Recipes for running SDS protein gel electrophoresis, SDS sample loading dye, and Coomassie staining protein gels.

5x SDS loading dye

• 125mM Tris-HCl, pH 6.75
• 20% (v/v) glycerol
• 10% (v/v) 2-mercaptoethanol
• 4% SDS
• 0.02% bromophenol blue

5x Running buffer (1L)

• 72 g glycine
• 15 g Tris-HCl
• 5 g SDS

Gel Stain

• 2.5g/L Coomassie Blue
• 45% (v/v) methanol
• 45% (v/v) glacial acetic acid

Gel Destain

• 10% (v/v) acetic acid
• 10% (v/v) methanol